1,2-Diarylethylenediamines as pre-column fluorescence derivatization reagents in high-performance liquid chromatographic determination of catecholamines in urine and plasma.

Simultaneous determination of 5-hydroxyindoles and catechols from urine using polymer monolith microextraction coupled to high-performance liquid chromatography with fluorescence detection

“…Using our proposed method, the concentrations of monoamines were measured in urine samples of different people (n = 3) (Table 7). Our 5-HIAA values are similar to those obtained previously by other researchers (2-50 M) [50,51] and our mean values for 5-HT, NE and E from urine samples from healthy volunteers are in good agreement with those obtained by other studies [52,53].…”

Simultaneous determination of catecholamines and their metabolites related to Alzheimer's disease in human urine

“…For example, Yoshitake et al determined serotonin, noradrenaline, DA, and their metabolites with FL detection following derivatization with benzyl amine and 1,2‐diphenylethylenediamine 25. Umegae et al used 1,2‐diarylethlenediamines as precolumn FL derivatization reagent to determine the CAs in urine and plasma 26. In this way, the method mentioned above could be applied to the analysis of biological samples.…”

Determination of serotonin, noradrenaline, dopamine and their metabolites in rat brain extracts and microdialysis samples by column liquid chromatography with fluorescence detection following derivatization with benzylamine and 1,2-diphenylethylenediamine

“…Recently, we have described several methods using discrete derivatization protocols based on either benzylamine or 1,2-diphenylethylenediamine (DPE) reagents for fluorescence detection of 5-hydroxyindoleamines and catecholamines, respectively [26][27][28][29][30][31]. These chemistries, though rather similar in terms of final reaction products (substituted benzoxazoles), differ mostly in reaction conditions (time and temperature) and the fluorescence yield, particularly for 5-HT and DA.…”