Determination of serotonin, noradrenaline, dopamine and their metabolites in rat brain extracts and microdialysis samples by column liquid chromatography with fluorescence detection following derivatization with benzylamine and 1,2-diphenylethylenediamine

Yoshitake, Takashi; Kehr, Ján; Yoshitake, Shimako; Fujino, Kaoru; Nohta, Hitoshi; Yamaguchi, Masatoshi

doi:10.1016/j.jchromb.2004.03.069

Cited by 109 publications

(70 citation statements)

References 51 publications

(51 reference statements)

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“…Various methods have been studied for the single determination of DA or PAR, for example, in detecting DA, HPLC [10], as fluorometry [11], UV-vis [12], and, in detecting PAR, spectrophotometry [13][14][15][16][17], near infrared transmittance spectroscopy [18], Fourier transform infrared spectrophotometry [19], spectrofluorometry [20,21], and chromatography [22,23]. However, the majority of these methods suffer from some disadvantages such as high costs, long analysis times, and requirement for sample pretreatment, and in some cases low sensitivity and selectivity that makes them unsuitable for routine analysis.…”

Section: Introductionmentioning

confidence: 99%

Development of a Method for a Sensitive Simultaneous Determination of Dopamine and Paracetamol in Biological Samples and Pharmaceutical Preparations

Babaei

Dehdashti

Afrasiabi

2011

International Journal of Electrochemistry

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A chemically modified electrode is constructed based on multiwalled carbon nanotube-modified glassy carbon electrode (MWCNTs/GCE). The measurements were carried out by application of differential pulse voltammetry (DPV), cyclic voltammetry (CV), and chronoamperometry (CA) methods. Application of DPV method showed wide linear range of DA from 1 μM to 540 μM and a detection limit of 0.098 μM (S/N = 3). The linear range of PAR of 3 μM to 300 μM and a detection limit of 0.15 μM, were obtained. The modified electrode showed electrochemical responses with high sensitivity, high selectivity, and excellent stability for DA and PAR determination at optimal conditions, which makes it a suitable sensor for simultaneous submicromolar detection of DA and PAR in solutions. The analytical performance of this sensor has been evaluated for detection of DA and PAR in human serum, human urine, and pharmaceutical preparation with satisfactory results.

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Section: Introductionmentioning

confidence: 99%

Development of a Method for a Sensitive Simultaneous Determination of Dopamine and Paracetamol in Biological Samples and Pharmaceutical Preparations

Babaei

Dehdashti

Afrasiabi

2011

International Journal of Electrochemistry

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“…However, the HPLC-FLD method requires the vigorous reaction conditions of heating to 50°C for 20 min for the derivatization, which are not desirable reaction conditions for biological samples. 14) Moreover, the HPLC-FLD method cannot determine 3-MT and HVA due to failure of the derivatization with fluorescent moiety. 3,14) In contrast to the previously reported methods, this derivatization method with ethyl chloroformate using the HPLC-MS system offers the advantages of less time required for the derivatization process and the ability to be performed in aqueous media.…”

Section: Application Of the Methods To Real Biological Samplesmentioning

confidence: 99%

“…14) Moreover, the HPLC-FLD method cannot determine 3-MT and HVA due to failure of the derivatization with fluorescent moiety. 3,14) In contrast to the previously reported methods, this derivatization method with ethyl chloroformate using the HPLC-MS system offers the advantages of less time required for the derivatization process and the ability to be performed in aqueous media. 33) In addition, this derivatization demonstrated satisfactory derivatization efficiencies with good yield in a short reaction time and enhanced data collection 34) because not only the levels of 5-HT, DA and their metabolites were simultaneously determined, but each peak area of 5-HT, DA and their metabolites was significantly expanded and more sensitive than those generated when using the HPLC-ECD method.…”

Section: Application Of the Methods To Real Biological Samplesmentioning

confidence: 99%

“…14) However, since this protocol requires the vigorous reaction condition of heating to 50°C for 20 min, it is necessary to develop a simpler method under mild reaction conditions. HPLC coupled with MS, which can be used to identify analytes simply on the basis of their m/z (mass-to-charge) ratios, 15,16) is a popular and powerful technique for analyzing neurotransmitters in several biological fluids.…”

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Simultaneous Measurement of Serotonin, Dopamine and Their Metabolites in Mouse Brain Extracts by High-Performance Liquid Chromatography with Mass Spectrometry Following Derivatization with Ethyl Chloroformate

Park

Myung

Kim

et al. 2013

Biological & Pharmaceutical Bulletin

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In order to measure the levels of serotonin (5-hydroxyltryptamine, 5-HT), dopamine (DA), 3,4-hydroxyphenylacetic acid (DOPAC), 3-methoxytyramine (3-MT) and homovanillic acid (HVA) simultaneously, an effective derivatization method followed by high-performance liquid chromatography (HPLC) coupled to electrochemical ionization mass spectrometry was used. The derivatization reaction of biological samples with ethyl chloroformate occurred rapidly at room temperature in aqueous conditions, and the resulting derivatives were isocratically separated with good selectivity using a C18 reversed-phase column within 30 min. The study results showed that the new derivatization procedure offers an excellent means of simultaneous determination of 5-HT, DA and their metabolites in mouse brain homogenates, which are important in a number of physiological and behavioral functions.Key words neurotransmitter; HPLC-MS; ethyl chloroformate; derivatization Dopamine (DA) and serotonin (5-hydroxyltryptamine, 5-HT), which are widely distributed as a monoamine neurotransmitter in the central nervous system (CNS), 1) play important physiological and behavioral roles such as the control and regulation of affective behavior, sleep, reward system, motor and cognitive functions.2) Changes of DA and 5-HT basal levels have been linked to some CNS-related disorders such as depression, panic disorder, schizophrenia and Parkinson's disease (PD).2,3) PD is pathologically characterized by the death of dopaminergic neurons in the substantia nigra pars compacta 4) and caused by neurotoxins such as 1-methyl-4-phenyl-1,2,3,6-tetrahypyridine (MPTP), which produces a clinical syndrome similar to PD. Because the administration of MPTP is known to affect the dopaminergic neurons in the nigrostriatal system with subsequent depletion of DA and its metabolites, 5,6) the measurement of 5-HT, DA and their metabolites is necessary to discover and test new drugs for treating these disorders. 7)Various analytical methods have been widely investigated for determining the level of monoamine neurotransmitters and their metabolites, including high-performance liquid chromatography (HPLC) coupled with electrochemical detection (ECD), dual ECD, fluorescence detection (FLD), FLD/ECD system, chemiluminescence detection and mass spectrometer (MS) and gas chromatography (GC) coupled with MS in biological samples such as plasma, rat brain, and mouse brain and in vitro experiments.8-10) HPLC-ECD, which is the most common analytical technique used to determine monoamines and their metabolites, has the advantages of low cost and fast speed 11) but maintaining its routine use is problematic due to sudden noise problems.12) The HPLC-FLD method is used to measure fluorescent derivatives of monoamine neurotransmitters by a derivatization procedure, 13) which was performed by benzylamine and 1,2-diphenylethylenediamine in a two-step reaction in weakly alkaline media and in the presence of potassium hexacyanoferrate (III).14) However, since this protocol requires the vigorous reaction ...

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“…Such sensitivity is virtually unattainable when using conventional electrochemical detection and ion-paired reversed-phase LC. The BA-DPE derivatization method was utilized for simultaneous determination of basal and pharmacologically stimulated levels of extracellular 5-HT, NE and DA sampled by microdialysis (Yoshitake et al, 2004b-d) and extracts from the rat brain (Yoshitake et al, 2004b). Chromatograms in Fig.…”

Section: Simultaneous Determination Of 5-ht Ne and Da Derivatized Wimentioning

confidence: 99%

Derivatization chemistries for determination of serotonin, norepinephrine and dopamine in brain microdialysis samples by liquid chromatography with fluorescence detection

Yoshitake

Kehr

Todoroki

et al. 2005

Biomedical Chromatography

102

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The present paper provides an overview on currently developed derivatization chemistries and techniques for determination of monoamine neurotransmitters serotonin (5-HT), norepinephrine (NE) and dopamine (DA) in microdialysis samples by microbore liquid chromatography with fluorescence detection. In mild alkaline conditions, 5-hydroxyindoles and catecholamines react with benzylamine (BA), forming highly fluorescent 2-phenyl-4,5-pyrrolobenzoxazoles and 2-phenyl(4,5-dihydropyrrolo) [2,3-f]benzoxazoles, respectively. However, for derivatization of DA a higher fluorescence intensity was achieved for reaction with 1,2-diphenylethylenediamine (DPE) rather than with BA, therefore for simultaneous determination of 5-HT, NE and DA in brain microdialysates, a two-step derivatization with BA followed by DPE was developed. The detection limits for 5-HT, NE and DA were 0.2, 0.08 and 0.13 fmol, respectively, in an injection volume of 20 microL, which corresponds to concentrations of 30, 12 and 19.5 pm, respectively in standard solution prior to derivatization. The experimental data presented demonstrate the ability of the technique to simultaneously monitor neuronally releasable pools of monoamine neurotransmitters in the rat and mouse brains at basal conditions and following pharmacological treatments or physiological stimuli. These techniques play an important role in drug discovery and clinical investigation of psychiatric and neurological diseases such as depression, schizophrenia and Parkinson's disease.

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Determination of serotonin, noradrenaline, dopamine and their metabolites in rat brain extracts and microdialysis samples by column liquid chromatography with fluorescence detection following derivatization with benzylamine and 1,2-diphenylethylenediamine

Cited by 109 publications

References 51 publications

Development of a Method for a Sensitive Simultaneous Determination of Dopamine and Paracetamol in Biological Samples and Pharmaceutical Preparations

Development of a Method for a Sensitive Simultaneous Determination of Dopamine and Paracetamol in Biological Samples and Pharmaceutical Preparations

Simultaneous Measurement of Serotonin, Dopamine and Their Metabolites in Mouse Brain Extracts by High-Performance Liquid Chromatography with Mass Spectrometry Following Derivatization with Ethyl Chloroformate

Derivatization chemistries for determination of serotonin, norepinephrine and dopamine in brain microdialysis samples by liquid chromatography with fluorescence detection

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