0294 Cardiovascular disease as a risk factor for disability retirement

Sá, Eduardo Costa; Torres, Rafael Augusto Tamasauskas; Rocha, Lys Esther

doi:10.1136/oemed-2014-102362.319

Cited by 1 publication

(1 citation statement)

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“…Cardiovascular disease (CVD) is a disease with a high rate of prevalence [1], disability [2], and mortality [3], which is a serious threat to human health. Up to now, CVD remains the first global cause of death, accounting for 17.3 million deaths per year.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive investigation on isolation, quantification, and activity evaluation of salvianolic acids for injection based on improved dry load injection technology

Gao

Zhou

Lele

et al. 2022

Separation Science Plus

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In this work, an effective method for simultaneous isolation of eight compounds from Salvianolic Acids for Injection was developed by preparative high‐performance liquid chromatography based on improved dry load injection. Firstly, the chemical structures of the obtained compounds were identified by mass spectrometry and nuclear magnetic resonance spectra. Subsequently, a sensitive liquid chromatography‐tandem mass spectrometry method was established to precisely quantify the eight isolated target analytes. The quantitative results show salvianolic acid B as the most abundant component (460.06–514.68‰) and protocatechualdehyde (0.03–0.08‰) as the least. Finally, the antioxidant activities of the isolated compounds were further investigated. The 2,2‐diphenyl‐1‐picrylhydrazyl‐scavenging assay indicated that (7′R, 8′S, 8″R, 8′′′R)‐epi‐salvianolic acid B had the lowest half‐maximal inhibitory concentration (5.05 μg/ml). This study reports on the development of an effective and rapid method for simultaneous isolation of multiple compounds from traditional Chinese medicine preparation, which could be used as reference compounds for further quality control and activities screening.

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Section: Introductionmentioning

confidence: 99%