Background: The “spring forward” change to Daylight Savings Time (DST) has been epidemiologically linked with numerous health and safety risks in the days following the transition, but direct measures of sleep are infrequently collected in community dwelling individuals. Methods: The Project Baseline Health Study (PBHS), a prospective, multicenter, longitudinal representative U.S. cohort study begin in 2017 launched a Sleep Mission in March 2021 to characterize sleep using patient-reported and wearable device measures, in free-living circumstances during the DST switch. Estimated sleep period duration, subjective restedness and quality, and watch metrics were compared before and after the DST transition during specified timeframes. Results: Of the total PBHS population of 2502 participants, 606 participants received an invitation and 419 participants opted in to the Sleep Mission (69.1%). The transition to DST resulted in both acute and lingering impacts on sleep. Acute effects included a 29.6 minute reduction in sleep period (p=0.01) and lower ratings of how well participants slept, as well as reduced next-day restedness. In the week following the time change, a persistent reduction in restedness scores, and a trend towards a decrease in sleeping heart rate variability were observed. Conclusion: The Daylight Savings Time transition is associated with an acute reduction in sleep period, and lingering impacts on self-reported restedness, as well as a trend towards reduced heart rate variability into the week following the transition. This work adds to a growing understanding of the persistence of impacts on sleep health metrics due to the DST transition.