Polycaprolactone (PCL) and biphasic calcium phosphate (BCP) have been considered as useful materials for orthopedic devices and osseous implants because of their biocompatibility and bone-forming activity. However, PCLbased scaffolds have hydrophobic surfaces reducing initial cell adhesion or proliferation. To overcome the limitation, we fabricated surface-modified PCL/BCP nanofibers using gamma-irradiation for bone tissue engineering. PCL/BCP nanofibers were prepared by electrospinning and then we supplemented hydrophilicity by introducing acrylic acid (AAc) through gamma-irradiation. We confirmed the surface of nanofibers by SEM, and then the initial viability of MG63 was significantly increased on the AAc grafted nanofibers, and alkaline phosphatase activity(1.239±0.226 nmole/µg/min) improved on the modified nanofibers than that on the non-modified nanofibers(0.590±0.286 nmole/µg/min). Therefore, AAc-grafted nanofibers may be a good tool for bone tissue engineering applications.
Englischer Begriff sucrase-isomaltase Definition Glykohydrolase der intestinalen Bürstensaummembran, die die Hydrolyse von Saccharose, Maltose, Isomaltose und a-Grenzdextrinen katalysiert. Beschreibung Saccharase-Isomaltase (Molmasse ca. 235 kDa) ist eine membranständige Glykohydrolase der intestinalen Bürstensaummembran insbesondere des Duodenums und des Jejunums. Das Enzym besteht aus 2 Untereinheiten mit jeweils unterschiedlicher katalytischer Aktivität, wobei die Saccharaseuntereinheit (EC 3.2.1.48) Saccharose zu Glukose und Fruktose, die Isomaltaseuntereinheit (EC 3.2.1.10) die a(1-6)glykosidische Bindung von Isomaltose und von a-Grenzdextrinen spaltet. Beide Untereinheiten spalten Maltose und am nicht reduzierenden Ende die a(1-4)glykosidische Bindung in a-Grenzdextrinen. Fehlende oder reduzierte Enzymaktivität bei der seltenen autosomal rezessiven hereditären Saccharase-Isomaltase-Defizienz führt zu Symptomen der Malassimilation mit Diarrhoe, Dehydratation und Gedeihstörungen.
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