This study was conducted to identify the recognition for nursing competency importance, nursing competency level, and their influencing factors of nurses in the long-term care hospitals. Participants were 243 nurses who were working in the 11 long-term care hospitals. Data were collected from August 25 to September 3, 2014. Data were analyzed using descriptive statistics, t-test, ANOVA, Pearson's correlation and multiple regression by SPSS 19.0. Mean scores of the recognition for nursing competency importance and nursing competency level were 4.21±0.48 and 3.47±0.46 respectively. Nursing competency level was significantly lower than the recognition for nursing competency importance. The variable influencing the recognition for nursing competency importance was the position(β=.19). The variables influencing the nursing competency level were the recognition of nursing competency importance (β=.37), age (β=.20), current work experience (β=.13), health status (β=.13). The results suggest the need of developing measurement tool and nursing competency enhancement programs which can well reflect the characteristics of nursing competency required in the long-term care hospital.
Antifreeze proteins (AFP) inhibit ice growth to permit the survival of polar organisms in the cold environments. The recombinant AFP from an Antarctic bacterium, Flavobacterium frigoris PS1, FfIBP (Flavobacterium frigoris icebinding protein), was produced using Pichia pastoris expression system. The optimum fermentation temperature (30 o C) and pH (5) for FfIBP production were determined using a fedbatch culture system. The maximal cell density and purified FfIBP were 112 g/L and 70 mg/L, respectively. The thermal hysteresis (TH) activity (0.85) of FfIBP obtained using a glycerol-methanol fed-batch culture system was 2-fold higher than that of the LeIBP (Leucosporidium ice-binding protein). This work allows for large-scale production of FfIBP, which could be extended to further application studies using recombinant AFPs.
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