This article surveyed the influence which 18 metal ions on phenol degrading bacterium XH-10 growth and the phenol degradation. , Mn 2+ at lower concentration to the phenol degrading bacterium XH-10 growth have promoter action, degrades the inhibitory action to be small, at high concentrations to the bacterium growth and the phenol degradation have obvious inhibitory; Ba 2+ the inhibitory action which degrades to the phenol is obvious, but at lower concentration have promoter action to the phenol degrading bacterium XH-10 growth; Fe 3+ the inhibitory action which growth to the bacterium is obvious, but at lower concentration have smaller inhibitory action to the phenol degrading bacterium XH-10 phenol degeneration. The results of the phenol degrading bacterium XH-10 for the treatment of wastewater containing phenol provides certain basis.Keywords: Phenol Degrading Bacteria XH-10; Heavy Metals Ions; Growth; Phenol Degradation 不同金属离子对苯酚降解菌 XH-10 的生长和苯酚降解的影响

A strain of medium-long chain n-alkanes degrading strain TY12 was screened from petroleum-contaminated soils of Nanchong oil refinery. We adopted several phenotypic and genotypical methods, such as morphological, physical and biochemical characteristics, antibiotic susceptivities, 16S rRNA gene sequences based phylogenetic analysis, to outline the basic biological characteristics and determinate the phylogenetic position. The Gram-negative isolate TY12 was a member of the genus Acinetobacter, short rods, no capsules, no endospores, catalase-positive, oxidase-negative, 0.9 -1.6 μm in diameter and 1.5 -2.5 μm in length; TY12 was susceptible to 14 kinds of tested antibiotics but resistant to Tetracycline, Amoxicillin, Ampicillin; The similarity between its 16S rRNA gene and that of its most closely related type strain in GenBank Acinetobacter beijerinckii CCM7266 (AJ626712), Acinetobacter beijerinckii LΜH6214 (AJ303013) were 100%. The optimal temperature and pH for the strain utilizing industrial ethanol were 30˚C and 7.0, and the optimal concentration of ethanol and concentration of yeast extract were 0.7% and 0.025 g/L, respectively. The n-dodecane degradation was 92% after the strain was growing on in hydrocarbon degradation medium with 1% (W/V) of n-dodecane at 30˚C and 180 r/min for 36 h. The strain could degrade a large range of n-alkanes with chain length C 12 -C 32 . It has potential in bioremediation of oil contaminated environment.Keywords: Petroleum Pollution; Hydrocarbon-Degrading Bacteria; Isolation and Identification; Acinetobacter

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