Adaptability of polymerase chain reaction (PCR) products to standard DNAs for real-time PCR quantification was evaluated by those standard curves. The threshold cycles (Ct values) of the same number of gene copies differed at -0.7-5.6 cycles between the genomic DNAs and the PCR products amplified from 16S rRNA genes of methanogens. This result indicates that the quantitative error occurs when PCR products are used as standard DNAs. Besides, the PCR efficiency (E) based on the slope of the standard curves was insufficient to evaluate the adaptability of PCR products as standard DNAs for real-time PCR quantification.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.