A 1,8-disubstituted pyrene-based fluorescent probe L was designed and synthesized using 1,8-diethynylpyrene and 8-aminoquinoline as raw materials by "click" reaction and the structure of L was characterized. The probe L exhibited good selectivity and sensitivity to Cu 2+ with fast response and good anti-interference ability in the THF/H 2 O (V∶V=1∶1, HEPES 1×10-2 mol/L, pH=7.4). Job's plot and high resolution mass spectrometry showed that stoichiometric ratio of L to Cu 2+ was 1∶1, and the density functional theory calculations indicated that L with Cu 2+ formed a multicomponent complex. In addition, the L-Cu 2+ showed highly selective sequential recognition of PPi with good sensitivity and fast response. The detection limit of L-Cu 2+ to PPi could reach 6.32 µmol•L 1 , which illustrated L-Cu 2+ had the potential application value.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.