The analysis of relevant and significant scientific research on the physiological and biochemical aspects of ketosis, and the main mechanisms of influence on reproductive function in this metabolic pathology is presented. Strengthening breeding potential and creating conditions for its implementation through modern feeding and maintenance technologies led to the spread of diseases of non-communicable etiology, including ketosis diseases. Ketotic diseases cause various disorders in carbohydrate-lipid, protein, water-electrolyte and vitamin-mineral metabolism. The monitoring of studies on the biological role of ketosis showed its effect on animal fertility, quality of female gametes and fetal development. When considering ketosis, it is important to understand precisely the mechanisms of influence, since many methods for diagnosing and combating ketosis diseases are based on their knowledge. The key mechanisms of ketone bodies formation, the reasons for increase in their concentration were considered. It is of great practical importance for development of diagnostic tests that make it possible to determine the direction of disturbances in energy and plastic processes. Based on the data presented in the studies, conclusions were drawn about the role of -hydroxybutyric acid as a direct factor in reducing the reproductive function of Bos taurus .
Проведено определение подвижности сперматозоидов в криоконсервированной сперме жеребцов (n=104) глазомерным (визуальным) методом и с помощью CASA (computer-assisted semen analysis), и определены различия в оценке подвижности сперматозоидов этими методами. В большинстве случаев оценка прогрессивной подвижности сперматозоидов в криоконсервированной сперме глазомерным методом была ниже, чем с помощью CASA. В 30% случаев визуальная оценка прогрессивной подвижности сперматозоидов жеребцов совпадала с оценкой CASA (±5%). Различия в оценке прогрессивной подвижности сперматозоидов разными методами в интервале от 0,1 до 15% отмечены более чем в половине случаев. При исследовании 31 (29,8%) образца данные визуального определения прогрессивной подвижности сперматозоидов оказалось значительно ниже, чем результаты оценки с помощью CASA, разница в оценке между этими методами составила от 15,1 до 42,5% сперматозоидов с прямолинейно-поступательным (прогрессивным) движением. The determination of sperm motility in cryopreserved stallion sperm (n=104) by eye (visual) method and by CASA (computer-assisted semen analysis) was carried out, and the differences were determined in the evaluation of sperm motility by these methods. In most cases, evaluation of progressive sperm motility in cryopreserved semen by eye method was lower than with CASA. In 30% of cases of visual assessment of progressive motility of stallion spermatozoa coincided with CASA assessment (±5%). Differences in the assessment of progressive sperm motility by different methods in the range from 0.1 to 15% were noted in more than half of the cases. In the study of 31 (29.8%) samples, the data of visual determination of progressive sperm motility was significantly lower than the results received using CASA, the difference in the evaluation between these methods ranged from 15.1 to 42.5% of sperm with progressive motion.
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