Bacterial cellulose (BC) is a biopolymer produced by different microorganisms, but in biotechnological practice, Komagataeibacter xylinus is used. The micro- and nanofibrillar structure of BC, which forms many different-sized pores, creates prerequisites for the introduction of other polymers into it, including those synthesized by other microorganisms. The study aims to develop a cocultivation system of BC and prebiotic producers to obtain BC-based composite material with prebiotic activity. In this study, pullulan (PUL) was found to stimulate the growth of the probiotic strain Lactobacillus rhamnosus GG better than the other microbial polysaccharides gellan and xanthan. BC/PUL biocomposite with prebiotic properties was obtained by cocultivation of Komagataeibacter xylinus and Aureobasidium pullulans, BC and PUL producers respectively, on molasses medium. The inclusion of PUL in BC is proved gravimetrically by scanning electron microscopy and by Fourier transformed infrared spectroscopy. Cocultivation demonstrated a composite effect on the aggregation and binding of BC fibers, which led to a significant improvement in mechanical properties. The developed approach for “grafting” of prebiotic activity on BC allows preparation of environmentally friendly composites of better quality.
Orally delivered probiotics must survive transit through harsh environments during gastrointestinal (GI) digestion and be delivered and released into the target site. The aim of this work was to evaluate the survivability and delivery of gel-encapsulated Lactobacillus rhamnosus GG (LGG) to the colon. New hybrid symbiotic beads alginate/prebiotic pullulan/probiotic LGG were obtained by the extrusion method. The average size of the developed beads was 3401 µm (wet), 921 µm (dry) and the bacterial titer was 109 CFU/g. The morphology of the beads was studied by a scanning electron microscope, demonstrating the structure of the bacterial cellulose shell and loading with probiotics. For the first time, we propose adding an enzymatic extract of feces to an artificial colon fluid, which mimics the total hydrolytic activity of the intestinal microbiota. The beads can be digested by fecalase with cellulase activity, indicating intestinal release. The encapsulation of LGG significantly enhanced their viability under simulated GI conditions. However, the beads, in combination with the prebiotic, provided greater protection of bacteria, enhancing their survival and even increasing cell numbers in the capsules. These data suggest the promising prospects of coencapsulation as an innovative delivery method based on the inclusion of probiotic bacteria in a symbiotic matrix.
The aim of this study was to increase productivity of Komagataeibacter xylinus C3 strain on nutrient media using industrial wastes as carbon source. By-product of sugar production, molasses, was static conditions, st strength -38.34 MPa and relative elongation at break -3.34%). The replacement of carbon source for molasses in MHS medium for BC production did not alter the polymer st st decrease of culture media by almost 2.8 times relating to MHS medium cost. bacterial cellulose, industrial waste, morphological properties, mechanical parameters, cost-The main object of study and practical use for the bacteria belonging to the family of Acetobacteriaceae [1-3]. Currently, 14 genera are present in this family: Acetobacter,
В обзоре проанализированацелесообразность применения пробиотиков и пребиотиков. Последние годы во всем мире наблюдается повышенный интерес к пробиотикам и пребиотикам и их использованию для сохранения и поддержания здоровья, а также лечения ряда заболеваний. Исследования пробиотиков дают основания предполагать наличия у них множество положительных свойств. Наиболее часто в качестве пробиотиков используются штаммы лакто- и бифидобактерий, которые являются достаточно безопасными даже для питания детей грудного и раннего возраста. Представлены пути, посредством которых пробиотики реализуют лечебный эффект. Показано, что они эффективны при многих заболеваниях, включая острые кишечные инфекции и другие воспалительные болезни кишечника, течение аллергического процесса, расстройства иммунитета. Нормальная микрофлора человека, нормофлора, выполняет ряд важных функций в поддержании жизнедеятельности организма. Защитная функция осуществляется в первую очередь бифидо – и лактобактериями, благодаря их способности подавлять патогенные бактерии и конкурировать с ними. Одним из важнейших функций микроорганизмов нормофлоры организма человека является иммуногенная стимуляция иммунной системы. Наиболее убедительные доказательства эффективности пробиотиков связанны именно с их использованием для улучшения функционирования кишечника и влияния на иммунную систему. The review analyzes the feasibility of using probiotics and prebiotics. In recent years, there has been an increased interest in probiotics and prebiotics and their use for the preservation and maintenance of health, as well as the treatment of a number of diseases around the world. Studies of probiotics suggest that they have many positive properties. Most often, strains of lacto- and bifidobacteria are used as probiotics, which are safe enough even for feeding infants and young children. The ways by which probiotics implement the therapeutic effect are presented. It has been shown that they are effective in many diseases, including acute intestinal infections and other inflammatory bowel diseases, the course of the allergic process, and immune disorders. Normal human microflora, normoflora performs a number of important functions in maintaining the vital activity of the body. The protective function is carried out primarily by bifidobacteria and lactobacilli, due to their ability to suppress pathogenic bacteria and compete with them. One of the most important functions of microorganisms of the normoflora of the human body is immunogenic stimulation of the immune system. The most convincing evidence of the effectiveness of probiotics is associated with their use to improve the functioning of the intestine and influence the immune system.
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