The paper highlights the problem of nitrogen fixation in a biological manner and considers prospects for the use of bacterial agents of an associative nature as an element of biotechnology in practice. Studies of promising domestic bacterial agents, based on associative nitrogen fixers, were carried out on zonal soils (chernozems) in the Rostov Region. The presented materials of scientific studies on preparative forms based on nitrogen-fixing microorganisms showed high performance, which was manifested in the progress of crop yields, and therefore these data can be used in agricultural production. Application of bacterial agents with nitrogenfixing species is profitable, since their cost is not high and the increase in additional crop production fully covers the costs and it cuts down the introduction of mineral nitrogen fertilizers. Nevertheless, it should be noted that the environmental aspect of bacterial agents' application consists in obtaining a clean source of nitrogen by cultivated plants and reduces pollution of the soil, atmosphere and water bodies.
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