Radical cystectomy with urine derivation in the segment of the small intestine is the "gold standard" for the treatment of muscle-invasive bladder cancer. The main method of urine derivation, providing the best quality of life for the patient, is an orthotopic bladder formed from the ileal segment of the intestine. The aim of study was to examine the effect of new chemical compounds on the contractile activity of the ileal wall of mini-pigs in vitro. Pyrrole-2-carbaldehyde 3-(dimethylamino) benzoylhydrazone and 1-methylisatin N, N-dimethylcarbamylhydrazone are two compounds with the greatest constrictor activity. Such compounds in concentration of 1×10 -3 mol / l, increase the contractile activity on 54.3 ± 9.2% and 51.2 ± 3.1%, the amplitude of contractions on 59.01 ± 12%, and 49.18 ± 14%, the frequency of reductions on 47.9 ± 6.4 % and 31.3 ± 5.3%. Pyrrole-2-carbaldehyde 2-hydroxybenzoylhydrazone and isatin benzoylhydrazone reduce hypertonicity on 49.6 ± 3.8% and respectively on 39.6% ± 3.1%. Pyrrole-2-carbaldehyde 2-hydroxybenzoylhydrazone and isatin benzoylhydrazone are the two compounds that reduce hypertonicity on 49.6 ± 3.8% and 39.6% ± 3.1%, making antispasmodic effect with tropicity to ileum.
Patients with malignant neoplasms of the bladder or severe anatomical and functional bladder abnormalities are the candidates for cystectomy and orthotopic urine diversion. The distal portion of the ileum is the most frequent anatomical organ, which is used for the formation of the urinary conduit. The formation of a urinary reservoir from the ileum that is unable to perform new functions, and mainly programmed to isolate enzymes, digest food and absorb nutrients, salts and minerals changes the energy exchange in the ileum wall under the influence of urine.
The aim of the research is the examination of energy metabolism changes in the ileum wall during the formation of artificial bladder in experimental conditions conducted on mini-pigs. Experimental studies were performed on 21 female mini-pigs with an average age of 6 to 14 months. The duration of the experiment was 12 months. An imbalance of the ATP-ADP-AMP system was observed in the transplant tissue of the graft in different postoperative periods. Changes in the level of ATP are characteristics of the development of tissue ischemia.
Results. The values of the adenosine nucleotides sum (the energy potential indicative of the respiration rate of mitochondria) and the phosphorylation index were lower at different times of observation in the transplant tissue. The energy charge value was lower to a lesser extent relative to the control.
Conclusion. The revealed changes in the level of adenosine nucleotides confirm the severity of the disturbances of the energy homeostasis in the transplant tissue at all stages of the observation.