Calcium phosphate bions (CPB) are biomimetic mineralo-organic nanoparticles which represent a physiological mechanism regulating the function, transport and disposal of calcium and phosphorus in the human body. We hypothesised that CPB may be pathogenic entities and even a cause of cardiovascular calcification. Here we revealed that CPB isolated from calcified atherosclerotic plaques and artificially synthesised CPB are morphologically and chemically indistinguishable entities. Their formation is accelerated along with the increase in calcium salts-phosphates/serum concentration ratio. Experiments in vitro and in vivo showed that pathogenic effects of CPB are defined by apoptosis-mediated endothelial toxicity but not by direct tissue calcification or functional changes in anti-calcification proteins. Since the factors underlying the formation of CPB and their pathogenic mechanism closely resemble those responsible for atherosclerosis development, further research in this direction may help us to uncover triggers of this disease.
An association between high serum calcium/phosphate and cardiovascular events or death is well-established. However, a mechanistic explanation of this correlation is lacking. Here, we examined the role of calciprotein particles (CPPs), nanoscale bodies forming in the human blood upon its supersaturation with calcium and phosphate, in cardiovascular disease. The serum of patients with coronary artery disease or cerebrovascular disease displayed an increased propensity to form CPPs in combination with elevated ionised calcium as well as reduced albumin levels, altogether indicative of reduced Ca2+-binding capacity. Intravenous administration of CPPs to normolipidemic and normotensive Wistar rats provoked intimal hyperplasia and adventitial/perivascular inflammation in both balloon-injured and intact aortas in the absence of other cardiovascular risk factors. Upon the addition to primary human arterial endothelial cells, CPPs induced lysosome-dependent cell death, promoted the release of pro-inflammatory cytokines, stimulated leukocyte adhesion, and triggered endothelial-to-mesenchymal transition. We concluded that CPPs, which are formed in the blood as a result of altered mineral homeostasis, cause endothelial dysfunction and vascular inflammation, thereby contributing to the development of cardiovascular disease.
Nanocomposites based on poly(styrene-block-isobutylene-block-styrene) (SIBS) and single-walled carbon nanotubes (CNTs) were prepared and characterized in terms of tensile strength as well as bio- and hemocompatibility. It was shown that modification of CNTs using dodecylamine (DDA), featured by a long non-polar alkane chain, provided much better dispersion of nanotubes in SIBS as compared to unmodified CNTs. As a result of such modification, the tensile strength of the nanocomposite based on SIBS with low molecular weight (Mn = 40,000 g mol–1) containing 4% of functionalized CNTs was increased up to 5.51 ± 0.50 MPa in comparison with composites with unmodified CNTs (3.81 ± 0.11 MPa). However, the addition of CNTs had no significant effect on SIBS with high molecular weight (Mn~70,000 g mol−1) with ultimate tensile stress of pure polymer of 11.62 MPa and 14.45 MPa in case of its modification with 1 wt% of CNT-DDA. Enhanced biocompatibility of nanocomposites as compared to neat SIBS has been demonstrated in experiment with EA.hy 926 cells. However, the platelet aggregation observed at high CNT concentrations can cause thrombosis. Therefore, SIBS with higher molecular weight (Mn~70,000 g mol−1) reinforced by 1–2 wt% of CNTs is the most promising material for the development of cardiovascular implants such as heart valve prostheses.
The role of acid treatment of Taunit carbon nanotubes in the formation of oxygen-containing functional groups on its surface as well as morphological and textural properties was studied. Acid treatment was carried out in an HNO3 solution or its mixture with H2SO4 under mild conditions (85 °C/1 h) with subsequent washing with distilled water or without washing. Properties of the initial and oxidized samples were investigated using elemental carbon, hydrogen, nitrogen, oxygen (CHNO) analysis, BET (Brunauer-Emmett-Teller) determination of surface area, X-ray diffraction, Raman and Fourier Transform Infrared Spectroscopy (FTIR) spectroscopy, and hydrogen temperature-programmed reduction. Treatment with HNO3 and HNO3/H2SO4 mixture was shown to be efficient for the formation of various oxygen-containing groups on the Taunit surface; therewith, the water washing step also contributed to functionalization of the surface. Depending on the oxidant, acid treatment increased graphite and oxygen content in the samples by a factor of 3‒4.5. Treatment with HNO3 without water washing exerted a weak effect on the graphite structure ordering, the concentration of aliphatic groups was high as compared to other oxidation conditions. Treatment of Taunit with the HNO3/H2SO4 mixture, on the contrary, increased the number of defects in graphite layers and decreased the concentration of aliphatic structures.
Most healthcare-associated infections (HCAIs) develop due to the colonisation of patients and healthcare workers by multidrug-resistant organisms (MDRO). Here, we investigated whether the particulate matter from the ventilation systems (Vent-PM) of health facilities can harbour MDRO and other microbes, thereby acting as a potential reservoir of HCAIs. Dust samples collected in the ventilation grilles and adjacent air ducts underwent a detailed analysis of physicochemical properties and biodiversity. All Vent-PM samples included ultrafine PM capable of reaching the alveoli. Strikingly, >70% of Vent-PM samples were contaminated, mostly by viruses (>15%) or multidrug-resistant and biofilm-producing bacterial strains (60% and 48% of all bacteria-contaminated specimens, respectively). Total viable count at 1 m from the ventilation grilles was significantly increased after opening doors and windows, indicating an association between air flow and bacterial contamination. Both chemical and microbial compositions of Vent-PM considerably differed across surgical vs. non-surgical and intensive vs. elective care units and between health facilities located in coal and chemical districts. Reduced diversity among MDRO and increased prevalence ratio in multidrug-resistant to the total Enterococcus spp. in Vent-PM testified to the evolving antibiotic resistance. In conclusion, we suggest Vent-PM as a previously underestimated reservoir of HCAI-causing pathogens in the hospital environment.
To develop new catalysts based on carbon nanomaterials with supported metal oxide nanoparticles for oxidative transformations of sulfur compounds, a series of metal oxide nanoparticle-decorated carbon nanotubes (MOx/CNTs) were prepared by incipient wetness impregnation at a variation of the active metal type (M = Ce, Mo, Cu). The thermal decomposition of bulk and CNT supported metal precursors used in the preparation of MOx/CNTs was analyzed under inert atmosphere employing several thermoanalytical techniques (thermogravimetry, differential thermogravimetry and differential scanning calorimetry) coupled with mass spectrometry. The thermolysis parameters of the bulk and supported metal precursors were compared and the effect of CNT support on the decomposition pattern of compounds was elucidated. It was established that the decomposition of metal precursors supported on CNTs was started and completed at temperatures of 15‒25 and 25‒70 °C lower, respectively, compared with the bulk active metal precursor. The enhancement of CNT support stability against thermal degradation is observed in the following row of metal cations: Ce < Cu < Мо < pristine and metal anions of precursor: nitrate < chloride < sulfate. The optimal mode of thermal treatment of catalyst and appropriate active metal precursors were selected for advanced synthesis of nanosized MOx/CNT catalyst.
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