Мета дослідження – вивчити причини фрагментації ДНК сперматозоїдів, встановити взаємозв’язок між порушенням ДНК та якістю еякуляту, а також вивчити вплив фрагментації ДНК сперматозоїдів на настання та виношування вагітності. Матеріали та методи. У дослідженні використано бібліосемантичний та аналітичний методи. Результати дослідження та їх обговорення. Під час виконання дослідження було проаналізовано 46 джерел сучасної зарубіжної літератури щодо фрагментації ДНК сперматозоїдів та її впливу на настання і виношування вагітності. Висновки. Аналіз літературних даних показав, що рівень фрагментації ДНК сперматозоїдів у межах 15–30 % є статистично значущим щодо зменшення позитивних результатів вагітності для in vivo, ВМІ, а також щодо підвищення ризику ранніх спонтанних викиднів. Якщо рівень фрагментації ДНК сперматозоїдів партнера >30 %, найефективнішим методом допоміжних репродуктивних технологій буде IVF або ICSI. Проте потрібно пам’ятати про ризик виникнення вад розвитку у плода, пов’язаних із цими процедурами допоміжної репродукції. Визначення рівня фрагментації ДНК можна використовувати як корисний аналіз у прогнозуванні викиднів, спричинених чоловічим фактором.
The content of flavonoids and xanthones in callus cultures derived from the roots of plants of six species of Gentiana L. genus was studied during the cultivation of these cultures in liquid growth media on foam substrates. The research findings indicate that for most callus cultures, which were grown on both agar and foam substrates, the content of biologically active substances (BAS) was higher or close to that in the roots of wild plants, but lower compared to their shoots. The content of flavonoids and xanthones in tissue cultures grown in liquid nutrient media exceeded (G. punctata, Mt. Breskul, G. asclepiadea, Mt. Pozhyzhevska, G. cruciata, Krenychi village and G. lutea, Mt. valley Rohnechska), was close (G acaulis, Mt. Turkul) or lower (G. cruciata, «Medobory» Nature Reserve, G. lutea, Mt. Troyaska) compared to those in the corresponding calluses on agar substrates. In the callus of G. pneumonanthe (Vyhoda village) during cultivation on agar medium and in liquid medium on foam substrates, flavonoids and xanthones were not detected. For most callus cultures of gentians: G. punctata (Mt. Breskul), G. asclepiadea (Mt. Pozhyzhevska), G. cruciata (Krenychi village), G. lutea (Mt. valley Rohnechska), cultivation in a liquid growth medium on foam substrates can increase the growth of callus biomass (1.3–1.7 times) and the content of flavonoids (1.2–1.6 times) and xanthones (1.2–2.3 times) in comparison with the same cultures on agar media. For the callus of G. cruciata («Medobory» Nature Reserve) and G. lutea (population of Mt. Troyaska) on liquid media with foam substrates, both the growth index by fresh weight and the content of secondary metabolites are lower compared to cultures from agar medium. The growth of callus G. acaulis (Mt. Turkul) on the nutrient medium with foam substrates is more intense than on agar, but with lowered BAS. Thus, the developed method of cultivating callus tissues of gentians in liquid nutrient media on foam substrates can reduce costs by replacing agar with foam substrates, as well as increase both the yield of biomass of most callus cultures and their ability to synthesize and accumulate flavonoids and xanthones. Key words: Gentiana L. species, in vitro cultures, foam substrates, flavonoids, xanthones.
Peculiarities of phytocoenotic association and and consortium relations of rare species of Gentiana lutea L., Gentiana punctata L., Gentiana acaulis L. are analyzed. All habitats of G. lutea populations are spatially associated with the bottoms of glacial cauldrons, krummholz with the species of Duschekia viridis (Chaix) DC, as well as herbaceous highland cenoses. These groups are autochthonous in terms of phytocenotic association of G. lutea. Most often, populations of G. lutea are part of the associations Pulmonario–Duschekietum viridis, Soldanello–Nardetum, in particular two of its subassociations: S.–N. gentianetosum, S.–N. narcissetosum, as well as subassociations Vaccinietum gentianosum, Calamagrostidetum gentianosum. Heavy pastoral load on highland cenoses led to the transformation of subassociations S.–N. gentianetosum and S.–N. Narcissetosum. Diagnostic species, Narcissus poeticus ssp. angustifolius (Curtis) Asch. et Graebn., G. lutea, in particular, have been replaced by dense-grained cereal Deschampsia cespitosa (L.) P. Beauv. The species of G. punctata tends to pine scrubs (Pinion mughi alliance), which are part of the alpine mat-grass (order Nardetalia), and also occur among subalpine shrub communities (Loiseleurio-Vaccinietea class), high-grass groups (Mulgedio-Aconitetea class) along the upper forest boundaries in riverbeds, descending into the forest belt (union Adenostylion alliariae). G. punctata is a diagnostic species of the endemic association Hyperico grisebachii – Calamagrostietum villosae and is a component part of the endemic associations Festucetum picturatae, Rhododendretum myrtifolii, a rare relict group of the ice age Centrario–Vaccinietum gaultherioides. The species composition of cenoses including G. punctata is also undergoing transformation affected by heavy pastoral load. In areas with heavy grazing, small clusters of G. punctata in the subalpine zone remain mostly in the gaps between the thickets of Pinus mugo Turra, D. viridis s and Juniperus communis subsp. nana. As in the case of G. lutea, the unfavourable species adjacent to G. punctata is D. saespitosa of high viability. Normal development of G. acaulis populations was found only in loosely coated cenoses comprised of Potentilla aurea L., Vaccinium myrtillus L., Festuca picturata Pils, Carex sempervirens Vill., Thymus sp., Anthoxanthum alpinum A. et D. Löve, as well as N. stricta and D. caespitosa (L.) Beauv. of low vitality. Adverse phytocenotic conditions for the growth of G. acaulis create species of D. viridis, Achillea submillefolium L., as well as N. stricta and D. caespitosa of high vitality. The consortia of the three species under study include 36 families of animals, with the varying degree of association: obligate (Apidae, Syrphidae, Formicidae, Diptera, Artropoda, Lumbricidae, Acariformes) and optional (Pieridae, Nymphalidae, Noridaidait, Gectuidae, Gectuidae, Chrysomelidae, Cantharididae, Alleculidae).
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