The article presents the results of the development of a rational method for obtaining an extraction preparation of shoots of Lespedeza bicolor, the results of determining the indicators of authenticity and quality in it, and the results of studying the anti-inflammatory effect. The extract preparation of shoots was obtained according to the well-known technological scheme: extraction of raw materials, purification of the extract, thickening of the extract. Ethyl alcohol of 70% concentration was chosen as the extractant, which is associated with the extraction of alcohol of this concentration of flavonoids - active biologically active substances of the raw material. The finished product was a thick liquid, greenish-brown in color with a specific odor. The yield of dry residue in the thick extract of shoots of Lespedeza bicolor was 22,2 ± 5,78%, the content of flavonoids, in terms of quercetin, was 1,98±0,027%. To study the local anti-inflammatory effect of the extract of the shoots of Lespedeza bicolor, studies were carried out to develop the composition and biopharmaceutical evaluation of a soft dosage form - an ointment with an extract of the shoots of Lespedeza bicolor. When biopharmaceutical evaluation of the release of the resulting ointment (gel) by diffusion through a semi-permeable membrane into an aqueous medium, it was determined that the maximum release of biologically active substances (flavonoids) from the gel is observed after 10 minutes. The introduction of an additional antimicrobial component, streptocide (sulfanilamide), into the composition of the developed gel with an extract of Lespedeza bicolor is substantiated. The study of the anti-inflammatory activity of gels with an extract of Lespedeza bicolor and gels of its combined form with streptocide was carried out on a model of acute exudative inflammation (formalin edema). It has been established that both the gel with the extract of the shoots of Lespedeza bicolor and the gel, containing the combination of the extract of the shoots of Lespedeza bicolor with streptocide, have been found to have anti-inflammatory activity; while the first exceeds the second gel in its activity, inhibiting inflammation more pronounced at the 3rd hour and after 24 hours, and is also more effective than the official drug - indomethacin ointment by 1.2-1.5 times, while remaining less toxic.
The article presents the results of a study of a polyphenolic extract of the herb Eupatorium cannabinum L., which grows on the territory of the Krasnodar Territory. The aim of the study was to conduct a phytochemical analysis of the BAS of a dry extract of Eupatorium cannabinum L. and determination of its antioxidant activity. Thus, as a result of the conducted studies, it was found that a diverse composition of phenolic compounds is identified in the dry extract of Eupatorium cannabinum L., among which the presence of rutin, quercetin salicylic, chlorogenic, caffeic, ferulic, π-coumaric and gallic acids was revealed. It was found that the content of phenolic carboxylic acids in the dry extract of Eupatorium cannabinum L. averaged 15.10%, flavonoids – 4.34%. The results of the studies conducted on the in vitro model system revealed the ability of the dry extract of Eupatorium cannabinum L. reduce the accumulation of Fe2+-induced POL products, which indicates the presence of direct antioxidant action. The mechanism of action of the resulting extraction product on the one hand may be related to the ability of phenolic compounds that make up the dry extract to act as free radical traps and restore reactive oxygen species, on the other hand, the resulting extract can act as chelators of Fe2+ ions, which significantly reduces the rate of free radical processes. The obtained data complement the previously discovered information about the antimicrobial effect of the dry extract of the cannabis herb and allow us to predict the potential effectiveness of the use of drugs based on it for the treatment of infectious and inflammatory skin diseases due to the presence of combined antimicrobial and antioxidant effects.
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