Aim. Investigation of cytokine status and the association between promoter regions polymorphism of interleukin-6, -10 and -18 genes in neonates with perinatal infections. Methods. Complex examination of 743 neonates with perinatal infections was conducted. The level of interleukin-1β, -6, -10, -18, and TNFα was measured with the use of standard method of enzyme-linked immunosorbent assay («sandwich» variant). Polymorphic variants of genes were analyzed with the methods of polymerase chain reaction and restriction fragment length polymorphism. Results. The results of the received data are indicative of the general tendency to increasing level of cytokines both in full-term and preterm infants with perinatal infections. Our study revealed the role of allelic variants at positions -174 G/C, -572 G/C, -597 G/A of interleukin 6, at positions -1082 G/C, -819 T/C of interleukin-10 and at positions -656 T/G, -137 G/C of interleukin-18 genes in the risk of development and progression of infections. It was determined that the presence of G allele at -174 and -572 positions of interleukin-6 gene, C allele at -819 and -137 positions of interleukin-10 and -18 genes, respectively, and G allele at -656 position of interleukin-18 gene was associated with the risk of perinatal infections in newborns. Conclusions. In neonates with perinatal infections imbalance of cytokine profile was revealed, that can be confirmed by increased level of pro-inflammatory cytokines (interleukin-1β, -6, -18, and TNFα) and decreased level of anti-inflammatory cytokine (interleukin-10) compared to uninfected neonates. Allelic variants of cytokine genes of interleukin-6 at position -572 G/C, interleukin-10 at position -819 T/G and interleukin-18 at position -652 T/G and 137 G/C are significantly associated with infectious diseases. Increased risk of prenatal infections is associated with genotypes GG of interleukin-6, TT of interleukin-10 and TT and GG of interleukin-18. Detection of haplotypes and haplogenotypes in the population of healthy and infected neonates revealed favorable haplotypes: GCC and GGG of interleukin-6, ATA of interleukin-10 and AGG and CGC of interleukin-18.
Infectious diseases in newborns are commonly intrauterine infections which affect greatly on the morbidity and mortality rates in neonates.Background: The purpose of this study was to analyse the neurological status, taking into account the neuroimmunological indicators
В структуре заболеваемости и смертности у новорожденных и детей первых месяцев жизни значительную роль играют врожденные инфекции. Внутриутробные инфекции относятся к особой категории заболеваний, которые при персистенции возбудителя имеют прогредиентное течение с хронизацией процесса при нарушении иммунной защиты и развитием тяжелых форм заболевания, инвалидизацией детей и высокой летальностью [4,7]. Внутриутробные инфекции, имеющие в 80% хроническое течение, являются причиной смерти недоношенных новорожденных в 68,1% случаев, доношенных в 49,7% [5].
Цель исследования. Изучение содержания нейронспецифической энолазы (NSE), белка S-100, активированной молекулы лейкоцитарной клеточной адгезии (ALCAM), «рецептора смерти» Death Receptor (DR5) в сыворотке крови у новорожденных детей с неонатальными судорогами. Материалы и методы. Проведено проспективное исследование 110 новорожденных с судорогами со сроком гестации от 38 до 42 недель. Результаты. В ходе проведенных исследований установлено, что уровень NSE у новорожденных с судорогами достоверно превышал концентрацию нейропептидов новорожденных контрольной группы в 3,1 раза; S-100 в 3,3 раза; ALCAM в 2,5, DR-5 в 2,1 раза по сравнению со здоровыми новорожденными. При проведении корреляционного анализа выявлены множественные связи с анамнестическими, клиническими, иммунохимическими показателями новорожденных с судорогами. Выводы. Экспрессия нейропептидов и факторов апоптоза у новорожденных на фоне гипоксически-ишемической энцефалопатии с нарушением проницаемости гематоэнцефалического барьера приводит к поддержанию судорожной активности и усугубляет дальнейший неблагоприятный неврологический исход. The purpose of the research. To study the content of neurospecific enolase (NSE), S-100 protein, activated leukocyte cell adhesion molecule (ALCAM), and Death Receptor (DR5) in the blood serum of newborns with neonatal seizures. Materials and methods. There was carried out a prospective study of 110 newborns with seizures with age stational age of 38 to 42 weeks. Results. As a result of the studies, there was found that the level of NSE in newborns with seizures reliably exceeded the concentration of neuropeptides in newborns of the Control Group by 3.1 times; S-100 by 3.3 times; ALCAM by 2.5; and DR-5 by 2.1 times compared with healthy newborns. Correlation analysis revealed multiple links with anamnestic, clinical, and immunochemical parameters of newborns with seizures. Conclusions. The expression of neuropeptides and apoptosis factors in newborns on the background of hypoxicischemic encephalopathy with a violation of the permeability of the blood-brain barrier leads to the maintenance of seizure activity and exacerbates further unfavorable neurological outcome.
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