Aim: To assess the feasibility and informative value of T-cell clonality
testing in peripheral T-cell lymphoma (PTCL). Patients and methods: Biopsies of
involved sites, blood, and bone marrow samples from 30 PTCL patients are
included in the study. Rearranged TCRG and TCRB
gene fragments were PCR-amplified according to the BIOMED-2 protocol
and analyzed by capillary electrophoresis on ABI PRISM 3130 (Applied
Biosystems). Results: TCRG and TCRB gene
clonality assay was valuable in confirming diagnosis in 97% of PTCL patients.
T-cell clonality assay performed on blood or bone marrow samples reaffirmed
lymphoma in 93% of cases, whereas morphological methods were informative in 73%
of cases only. We observed multiple TCRG and TCRB
gene rearrangements, loss of certain clones in the course of the
disease, as well as acquisition of new clones in 63% of PTCL cases, which can
be attributed to the genetic instability of the tumor. Conclusion:
TCRG and TCRB gene clonality assay is
beneficial for the diagnosis of PTCL. However, the presence of multiple clonal
rearrangements should be considered. Clonal evolution in PTCL, particularly
acquisition of new clones, should not be treated as a second tumor. Multiple
TCRG and TCRB gene rearrangements may
interfere with minimal residual disease monitoring in PTCL.
Transcutaneous two-wave pulse oximetry is the most popular and prevalent method for studying blood oxygenation. However, during its implementation, smokers do not take into account the level of carboxyhemoglobin, which leads to an erroneous overestimation of hemoglobin saturation with oxygen. The computer program developed by us makes it possible, without the use of additional diagnostic equipment, to correct the results of monitoring blood oxygenation for the level of carboxyhemoglobin, correcting the indicated diagnostic inaccuracy in assessing the saturation of hemoglobin by oxygen in smokers.
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