Background. Research interest in ceruloplasmin (CP) has significantly increased in recent years owing to new discoveries of its properties including anti-inflammatory and anti-oxidant effects. Data on CP blood plasma level in patients with herpetic infection caused by herpes simplex virus are scarce and often contradictory. Most point to a reduction of CP in the blood plasma of patients during the exacerbation of the disease with gradually return to normal values after treatment. There is evidence of an increase in CP levels during the acute period of CRHI (chronic recurrent herpes infection) and decrease in remission.Materials and methods. The content of ceruloplasmin in blood plasma was determined by immunoturbidimetry using the test-systems "Spinreact" (Spain) and biochemical analyzer "Architect C8000".Results. We found that in patients with severe forms of chronic recurrent herpes infection in the exacerbation period, CP levels were increased by approximately 35% relative to the control values. However in the remission period in 80% of patients, CP concentrations remained elevated and in some patients the CP level was even increased in comparison with values in the exacerbation. Such dynamics of CP were not caused by the exacerbation of concomitant diseases.Conclusion. The increased CP levels in the remission period in patients with severe forms of chronic recurrent herpes infection may have been caused by its effect as an endogenous antioxidant. No correlations of CP levels with other laboratory signs of acute inflammation were found but identified was a relation to free radical activity.
The genes of the major histocompatibility complex (HLA) play a significant role in the genetic predisposition to juvenile idiopathic arthritis (JIA) and determine up to 18 % of disease risk. This work was aimed to reveal associations of the HLA characteristic pattern with two clinically different forms of JIA in the Belarusian population. 24 patients diagnosed with systemic JIA, 24 patients with oligoarticular JIA and 24 healthy controls were included into the study. High-throughput HLA typing for 11 loci was performed using TruSight HLA v2 Sequencing Panel (Illumina) on the MiSeq system. DQB1*04:02:01 (p = 0.026; OR 5.88 [1.20–28.72]), DRB1*08:01:01 (p = 0.07; OR 3.94 [1.01–15.39]) and DR8-haplotype (p = 0.006; OR 3.95 [1.34–11.63]) frequencies were significantly higher in patients with oligoarthritis but not systemic JIA when compared with controls. While DQA1*05:01:01 and DQB1*02:01:01 alleles showed a protective effect against both systemic (p = 0.007, OR 0.08 [0.009–0.65]; p = 0.01, OR 0.09 [0.01–0.83]) and oliarticular JIA (p = 0.026, OR 0.16 [0.03–0.79]; p = 0.046, OR 0.2 [0.04–1.00], the negative association of the DRB1*03:01 allele was revealed only for systemic JIA (p = 0.03, OR 0.11 [0.01–0.88]). Thus, the obtained results suggest that different HLA-patterns are involved into the formation of various JIA subtypes.
The concentration of ceruloplasmin in plasma was studied in 57 patients with severe form herpes infection. Augmentations of ceruloplasmin were observed in the periods of remission and exacerbation of the disease, but not exit out of referents significance. At the passage herpes infection of exacerbation in the remission of the disease concentration of ceruloplasmin was observed. In patients in the periods of remission with herpes infection correlations between the ceruloplasmin concentration and lipid peroxidation as well as superoxiddismutasa levels were observed, which were absent in control group.
The functional activity of neutrophils and lipid peroxidation was studied in 73 patients with severe form of herpes infection. The augmentation of first, second (trienic conjugates) and last (Schiff bases) products of lipid peroxydation in plasma and in erythrocytes was increased in the periods of remission and exacerbation of the disease. The increasing degree of Schiff bases was higher in the remission periods than in the exacerbation periods of the disease. The oxygen production of neutrophils was increased in the periods of remission and exacerbation of the disease.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.