The genus Allium L. (Amaryllidaceae), the most numerous among monocotyledonous plants, includes such economically important vegetable crops as onion (A. cepa), garlic (A. sativum) and leek (A. porrum). Leek has a high taste and proven valuable dietary properties and is one of the most popular vegetable crops in Western Europe. Despite a high importance of leek as a vegetable, this species is rarely the subject of molecular genetic studies. The genetic diversity of leeks has never been studied before. Therefore, in this work, we studied the nuclear variability (AFLP) and the chloroplast (nucleotide sequence analysis) genomes using a broad sample. For this work, 65 leek accessions were selected from the collection of the Scientific Center of Vegetable Crops, which included varieties of domestic and foreign breeding. As a result of an AFLP analysis and processing of the DNA spectra obtained, 760 fragments were identified, of which 716 were polymorphic for the leek accessions being analyzed. The calculated genetic distances between the leek samples varied from 0.4 to 0.76, which is comparable to the intraspecific polymorphism of related Allium species (onions, garlic). Analysis of the genomic structure with STRUCTURE 2.3.4 divided the leek samples into seven groups, which generally coincides with the clustering of these samples. To assess the variability of the chloroplast genome, nine sites of the chloroplast genome were sequenced in the leek samples, both non-coding (intergenic spacers rpl32-trnL, ndhJ-trnL, and intron rps16 gene), and protein coding genes (psaA, psaB, psbA, psbB, psbE, petB). The analysis of the sites of the leek chloroplast genome revealed an extremely low level of their polymorphism, only six SNPs were detected in the studied sequences with a total length of about 10,500 bp. Thus, as a result of this work, a high level of polymorphism of the leek nuclear genome was revealed, while the polymorphism of the chloroplast genome was extremely low.
Sequencing and comparative characterization of plant plastid genomes, or plastomes, is an important tool for modern phylogenetic and taxonomic studies, as well as for understanding the plastome evolution. The genusAlliumL. (family Amaryllidaceae) incorporates more than 900 species, includes economically significant vegetable crops such as garlicA. sativum, onionA. cepa, leekA. porrum, etc. In this work, the plastome of garlicA. sativumhas been completely sequenced. TheA. sativumplastome is 153172 bp in size. It consists of a large unique (LSC, 82035 bp) and small unique (SSC, 18015 bp) copies, separated by inverted repeats (IRa and IRb) of 26561 bp each. In the garlic plastome, 134 genes have been annotated: 82 protein-coding genes, 38 tRNA genes, 8 rRNA genes, and 6 pseudogenes. Comparative analysis ofA. sativumandA. cepaplastomes reveals differences in the sizes of structural elements and spacers at the inverted repeat boundaries. The total numbers of genes inA. sativumandA. cepaare the same, but the gene composition is different: therpl22gene is functional inA. sativum, being a pseudogene inA. cepa; conversely, therps16gene is a pseudogene inA. sativumand a protein-coding gene inA. cepa. In theA. sativumandA. cepaplastomes, 32 SSR sequences have been identified. More than half of them are dinucleotides, and the remaining are tetra-, penta-, and hexanucleotides at the same time, trinucleotides were absent. The compared plastomes differ in the numbers of certain SSRs, and some are present in only one of the species.
Relevance and methods. Losses of agricultural crops are associated not only with the development of diseases during the growing season, but also during post-harvest storage. Garlic is a popular vegetable and aromatic crop in world. Significant losses in garlic yield during cultivation and storage are associated with fungal pathogens, the most harmful of which are representatives of the genus Fusarium. In the Moscow region, the defeat of garlic by Fusarium occurs annually, but with varying intensity. At the Federal Scientific Vegetable Center (FSVC), it was shown that rot and wilting of garlic plants is caused by a complex of pathogenic fungi, including mainly different species of Fusarium. At the same time, the ratio of Fusarium species in the pathogenic complex changes from year to year, new Fusarium species and their races are registered. The aim of this study was to identify fungal phytopathogens causing dry rot of garlic cloves during post-harvest storage. To carry out the work, garlic bulbs of cultivars Dubkovsky and Strelets were taken from the FSVC storage.Results. As a result of visual examination, cloves with symptoms of dry rot were identified. The diseased cloves tissues were plated on potato dextrose agar to obtain fungal colonies. Analysis of the morphological and cultural characteristics of fungal isolates, as well as the nucleotide sequences of four DNA regions (ITS spacers, genes EF1α, RPB1, and RPB2) showed that the causative agent of dry rot of garlic cloves is the pathogenic fungus Fusarium proliferatum. In addition, in the field, identification was carried out based on the analysis of the sequences of spacers ITS and the EF1αgene of phytopathogenic fungi inhabiting the root zone of garlic plants. As a result, two species of fungi of the genus Fusarium (F. proliferatum and F. oxysporum f. sp. cepae), as well as the species Rhizoctonia solani, Volutella rosea, and Ceratobasidium sp. were found in the root zone of garlic cultivars.
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