Aim: to investigate a fecal microbiota composition and to identify candidate biomarkers of celiac disease (CD) by serum metabolomics analysis. Methods: the quantitative real-time polymerase chain reaction was used for fecal microbiota assessment. Serum metabolomic assays were conducted using the GC-MS. Results: serum of CD patients showed significant increases in stearic acid, 2-HIVA, succinate, fumarate and benzoate compared to HC. A decrease in the level of eicosadiene and an increase in AA in blood were determined. The ratio of AA to EDA was statistically significant (4.84 vs. 3.28, p=0.033). The elongase activity index in patients with celiac disease tended to increase (p=0.067). The colon microbiome in CD was characterized by decreasing in the level of butyrate-producing Faecalibacterium prausnitzii (F.p.) and Bifidobacterium spp.. Significant negative correlations were observed; between the levels of Bifidobacterium spp. and F.p. and the concentration of succinic acid (rs= =-0.343 [p=0.026] and rs=-0.430 [p=0.005], respectively); the F.p. and the fumaric acid (r=-0.429, p=0.005); the benzoic acid and the amount of Bifidobacterium spp. (r=-0.341, p=0.025). Conclusion: significant changes in serum levels of microbial and endogenous metabolites, reflecting some metabolic pathways disturbances were observed in CD. Metabolites and metabolomic index reflecting the balance between pro-inflammatory and anti-inflammatory components, may be considered as candidate biomarkers of chronic inflammation and metabolic dysbiosis in CD. An increased B. fragilis/F.p. ratio can serve as available biomarker for intestinal pro-inflammatory dysbiosis in CD.
Орешко Аркадий Юрьевич, преподаватель института остеопатии Мохов Дмитрий Евгеньевич, д. м. н., заведующий кафедрой остеопатии; директор Института остеопатии Орешко Людмила Саварбековна, д. м. н., профессор кафедры пропедевтики внутренних болезней, гастроэнтерологии и диетологии им. С. М. Рысса Трегубова Елена Сергеевна, д. м. н., профессор кафедры остеопатии; доцент Института остеопатии Селиверстов Павел Васильевич, к. м. н., доцент кафедры внутренних болезней и нефрологии Семенова Елена Анатольевна, к. м. н., ассистент кафедры пропедевтики внутренних болезней, гастроэнтерологии и диетологии им. С. М. Рысса
The article presents a modern view of celiac disease within the framework of the classification concept of gluten- associated disorders. The prevalence of the disease, the modern model of the etiopathogenesis of celiac disease, clinical manifestations, and the possibilities of differential diagnosis are discussed. According to the European guidelines, a strategy for monitoring outpatients with celiac disease is presented, based on baseline characteristics of the disease, regular doctor- patient interaction, and prevention of gluten- associated disorders.
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