Introduction. One of the promising methods of treating oncological diseases is high-frequency focused ultrasound (HIFU). The high-frequency acoustic field generated by the emitters induces mechanothermal effects whose significance has not yet been fully assessed and their study will change the tactics of therapy. To analyze their role, we selected cellular perivascular regulators of local blood flow – mast cells (MCs). Changes in the microcirculation (MCT) allow us to judge the morphofunctional state of the MC. The aim of the study is to assess the state of the rat’s skin periphocal tissue blood flow and MCs morphofunctional properties after HIFU-exposure.Material and methods. Adult male Wistar rats were used. Animals were divided into 3 groups: control, non-contact local heating of the skin and HIFU-induced exposure. For HIFU, an experimental setting was used, consisting of a concave emitter H-148 S / N 010 with a central oscillation frequency of 1.4 MHz. In the averaged interference spot, the radiation intensity was 8.2 kW/cm2. The exposure duration from 150 to 700 ms. Depth of focus is about 1 mm. To assess the MCT, a laser doppler flowmeter was used. Material for the manufacture of film preparations of MCs was taken at the site of exposure and according to the standard method. MCs calculation was carried out using the software «Multimedia Catalog» taking into account their morphofunctional characteristics.Results. HIFU exposure led to a significant increase in blood flow in rat’s skin at an exposure time of 250 ms or more (p<0.01). The maximum increase in perfusion was noted at 400 ms. The highest increase in tissue blood flow with local heating was observed at 45°C (p<0.001). HIFU-exposure led to a significant decrease in the density of MCs in the subcutaneous fat tissue of rat’s skin compared with the control at an exposure time of 250 ms or more (p<0.05) and a significant decrease in the relative number of type I cells, and an increase in type II and III, which indicates degranulation (p<0.05). Similar changes in the amount and morphofunctional characteristics of MCs were observed in a series of experiments with local heating, but the changes were not so significant.Conclusions. With HIFU-induced exposure, increased MCT in the peripheral zones is observed, accompanied by processes of morphofunctional rearrangement of the MCs, which indicates a combined mechanothermal disturbance in the focus zone, which forms the spatiotemporal continuum of the thermal field.
Ciliates is a promising object for the accumulation and distribution studies of new photosensitizers in the cell by laser confocal scanning microscopy. Data of Radachlorin, Bengal rose and Coproporphyrin III in the cells is shown.
Introduction. Rosacea is a chronic inflammatory dermatosis characterized by the presence of pathogenesis of inflammation and vascular changes caused by exposure to antimicrobial peptides (LL-37) and toll-like receptors (TLR2). It has been established that the pathogenetic link is a target for a new therapy of rosacea.Objective – to evaluate the potential combined effect of local Ivermectin 1 % cream and a pulsed dye laser (PDL) with a wavelength of 595 nm on a model of rosacea-like inflammation on mice after 4-fold introduction of recombinant antimicrobial peptide LL-37.Material and methods. Adult female BALB/c white mice weighing 23.5±0.66 g were used, divided into 4 groups: control; group 2 (biodistillate water was introduced in a volume of 40 μl, within 48 hours); group 3 (without treatment, intracutaneously injected paravertebrally into the lumbar region 40 μl of peptide LL-37 (320 μM), 4 times every 12 hours); group 4 (with treatment, 1 % Ivermectin cream was applied after each peptide injection, 60 hours after the first injection, the PDL device with a wavelength of 595 nm was treated, the area irradiated with a surface of 50 mm2).Results. The result indicates that the local injection of LL-37 into the skin of mice caused a dramatic expansion and fullness of the vessels of both the arterial and venous link. The integrity of the walls of the blood vessels of the microcirculatory bed in the dermis and the hypodermis has broken, resulting in multiple small hemorrhages in the tissues. In the remaining vessels, the endothelium was swollen, the adventitia was infiltrated with leukocytes. The number of mast cells increased significantly, but only some of them degranulated.Conlusions. As a result of the combined local anti-inflammatory and laser effects, leukocyte infiltration of the dermis and hypodermis decreased, in the dermis a part of the vessels was desolate, the endothelium in them was swollen. Enlarged full blood vessels remained in the hypodermis, the endothelium was absent in them, insignificant adventitia infiltration remained. The proposed combination of effects, taking into account the morphological picture, is promising and requires additional study in patients with rosacea without the use of invasive research methods.
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