Recombinant human adenovirus serotype 5 (Ad5/35F-IL2) with
modified fibres containing the C-terminal domain fiber-knob of human adenovirus
serotype 35, carrying the gene of recombinant human IL-2, has been designed. As a
result of the fiber modification, the adenovirus can efficiently deliver the genetic
information to bone marrow leukocytes and the tumor blood cells KG-1A (human
myeloblastic leukemia cells) and U937 (human histiocytic lymphoma cells), which are
normally resistant to Ad5 infection. The flow cytometry data reveal that the
modified Ad5/35F penetrates into a population of monocytes, granulocytes, and blast
cells of human bone marrow. The expression of interleukin-2 in CAR-negative bone
marrow leukocytes (3682.52 ± 134.21 pg/ml) and the cell lines KG-1A (748.3 ± 32.8
pg/ml) and U937 (421.5 ± 59.4 pg/ml) transduced with adenovirus Ad5/35F-IL2 is
demonstrated. The fiber-modified adenovirus can be used as a vector for the
efficient gene delivery of interleukin-2 to human normal and tumor hematopoietic
cells.
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