Object of development: database «Strains of coagulase-negative staphylococcus, persisting in hospitals of different profile».The purpose of the study: inclusion of modern information technologies in the system of epidemiological surveillance and monitoring of infections caused by ecovars of coagulasenegative staphylococcus circulating in Nizhny Novgorod. Methodology: source data was converted into a set of related tables and organized into a hierarchical two-tier database. The database contains information about 1045 strains, circulated in hospitals of different profile of Nizhny Novgorod. To form a sample describing of clinical, epidemiological and phenotypic characteristics of strains requests are made. As a result of the query, a table is formed, where each line corresponds to one strain and contains information about the date, source and location of the recovery, the phenotype of antibiotic and phage resistance, the presence of the mecA gene, as well as sensitivity to different classes of disinfectants and the ability to form biofilm. The database is refillable. It was created using Microsoft Office Access with a standard application package. The database includes 17 queries and 14 tables. Type of implementing computer: IBM PC-compatible PC. View and version of the database management system: Access 2002 and later. Operating system version: Windows 7 and later. The developed database allows searching and processing of epidemiologically significant information about strains of coagulaze-negative staphylococcus.The application of the database is to improve the microbiological monitoring system in epidemiological surveillance of healthcare-associated infections.
Numerous studies have proved that microscopic fungi take part in the pathogenesis of various human diseases, so micromycetes in the air of indoor spaces are considered as a factor that negatively affects to human health. Concentration in medical institutions sick persons pertain to groups of high risk of development of opportunistic infectious diseases dictates the need of conducting control of mycetogenic contamination of rooms. Purpose of the study: research of quantitative structure and specific variety of a mykobiota of the department’s air in one of health care centers of Nizhny Novgorod. Materials and methods. Sampling of air was made by an aspiration method on Petri dish with solid mediums. Then was defined the maintenance of colony forming units (CFU) in 1000 liters of air. Specific identification of the marked-out cultures was carried out by classical bacteriological methods on their morphological and cultural features. Results. It was established that in air of the surveyed departments 32 various species of the mold fungi belonging to the class Hyphomycetes in total circulates. At assessment of specific structure it was established that representatives of two species – Aspergillus niger and Penicillium simplicissium most often met. Except them Alternaria longipes, Fusarium gibbosum, Cladosporium elatum were most often allocated. Conclusion. The conducted researches have shown that in air of the surveyed departments the microscopic fungi relating to pronounced aeroallergens are circulate. The obtained data can serve as a prerequisite for development of a microbiological method of assessment of a sanitary condition of health care centers and degree of their potential health hazard to patients.
We researched the sensitivity to disinfectants and the ability to form a biofilm of coagulase-negative staphylococci circulating in a children’s hospital in Nizhny Novgorod. It is shown that the most common resistance to disinfectants and a high degree of biofilm formation was observed in S. haemolyticus cultures. A comparison of testing sensitivity to the Avansept disinfectant planktonic cells of coagulase-negative staphylococci and the same strains in the form of a biofilm was performed. It was found that the resistance to «Advance» significantly increases in strains grown in the form of biofilm compared with plankton cultures.
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