Studies were conducted at the experimental station of the Stavropol State Agrarian University on leached chernozem, powerful, low-humus heavy loam on loess-like loam in 2017. The goal was to study the effect of the introduction of rocks rich in chemical composition (limestone-shell rock, apatite and phosphogypsum), both separately and jointly, on the microbial phase of the soil. The determination of the number of microorganisms was produced on dense nutrient medium by direct counting of colonies. It was found that the amount of ammonifiers under the control was 37 ml CFU g -1 (colony-forming units), increasing 1.3 -1.5 times with separate use and 2.5 -3 times with the joint use of rocks. Similar changes were observed with respect to the number of nitrifiers and aerobic nitrogen fixers of the type Azotobacter. The number of cellulose-depleting microorganisms in the remineralization variants reached 220,00 -230,00 CFU g -1 compared to 115,00 CFU g -1 under the control. With the introduction of separate rocks, there was a decrease in the occurrence of pathogens, while with a joint introduction they were not detected. The frequency of occurrence of toxin formers, such as Aspergillus and Penicillium, reached 100% at the control and decreased by 20 -40% at the experimental variants. It was revealed that the introduction of shell limestone, apatite and phosphogypsum had an effect on the increase in the number of soil microbiota of various physiological groups. Among the fungal microflora, the number of pathogens and toxin formers decreases and the number of pathogen antagonists increases.
The following field experiences were performed on the Chernozem southern carbonate: 1.Control, 2. Sulphoammophos – 150 kg/ha, 3. Sulphoammophos – 250 kg/ha, 4.Phosphogypsum – 3 t/ha, 5.Phosphogypsum – 6 t/ha, 6.Phosphogypsum – 12 t/ha, 7. Phosphogypsum – 3 t/ha ammophos –70 kg/ha ammonium nitrate, 100 kg/ha 8. Phosphogypsum – 6 t/ha ammophos -70 kg/ha ammonium nitrate, 100 kg/ha 9. Phosphogypsum – 12 t/ha ammophos–70 kg/ha ammonium nitrate, 100 kg/ha. 10. ammophos-70 kg/ha + ammonium nitrate 100 kg/ha. The determination of the number of microorganisms produced in the phase of milky-wax ripeness of winter wheat on the selective nutrient medium: meat-peptone agar – number of ammonifiers; on starch and ammonia agar – microorganisms that assimilate mineral forms of nitrogen on the Hutchinson medium – calculatorsreal the number of microorganisms on the Czapek-Dox medium – the number of micromycetes on the Ashby medium – the number of aerobic nitrogen-fixing bacteria of the genus Azotobacter. Introduction phosphogypsum, sulphoammophos, ammophos and ammonium nitrate contributes to the increase in the numbers of various physiological groups of microorganisms. The highest values of the studied indicators in comparison with the control was achieved through the joint application of phosphogypsum – 12 t/ha ammophos-70kg/ha and ammonium nitrate-100kg/ha: ammonifiers on 123.4 million CFU/g (or 2.5 times); nitrifiers at 138 million CFU/g (3.3 times), microscopic fungi on 90,0 thousand CFU/g (1.7 times); cellulose-fermenting microorganisms on 250.6 thousand CFU/g (more than 2 times); aerobic nitrogen-fixing bacteria of the genus Azotobacter by 30.7 thousand CFU/g (1.5 times). Thus, the most responsive to the introduction of phosphogypsum and fertilizers microorganisms that convert mineral and organic nitrogen compounds.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.