We compared anxiety, neuromotor, and cognitive functions in mutant rats with different allelic variants of dopamine transporter DAT knockout receiving balanced or excess in fat and fructose diet. The experiments were performed in DAT −/− homozygotes, DAT +/− heterozygotes, and DAT +/+ wild type rats. The genotype of DAT ‐ KO rats was confirmed by restriction analysis of DAT gene compared to behavioral responses in the open field test ( OF ). Animals in the first groups of each strain were fed a balanced AIN 93M diet; and those in the second groups with a high‐fat/high‐fructose diet. Neuromotor function was studied as grip strength, and behavioral responses were assessed in the elevated plus maze and conditioned passive avoidance response tests. The mass of the internal organs and white and brown fat, as well as selected lipid and nitrogen metabolism parameters in blood plasma were determined at the end of the experiment. DAT −/− had the highest specific grip strength, and showed an increase in initial exploratory activity in comparison with DAT +/− and DAT +/+. The exploratory activity was significantly reduced in the second test compared to the first one in DAT −/− and DAT +/− of first but not second group. Anxiety decreased with age in the second groups of DAT +/− and DAT +/+ (but not in DAT −/−) and was higher in DAT +/+ than in DAT +/− and DAT −/−. Excess fat and fructose resulted in the deterioration of short‐term memory in DAT +/+. Lipidomic indices of blood plasma were less responsive to diet in DAT −/− and DAT −/+ in comparison to DAT +/+. The increased As AT /Al AT activity ratio in DAT −/− compared with those in DAT +/+ suggests the activation of catabolism activity in the mutants. The consumption of excess fat and fructose significantly modified the effects produced by DAT gene allelic variants presumably due to the influence on the processes of dopamine metabolism.

In vitro production of immunoregulatory cytokines (IFN-α, IL-31, TNF-β, IL-17A, IL-7, IL-1RA, IL-1α, IL-10, IL-15, IL-21, IL-22, IL-23, IL-27, and IL-9) by dendritic cell cultures was compared in ski athletes and healthy donors. Effect of prolonged intense physical exercise on secretory activity of immune cells was investigated. In both groups, secretion of IL-1RA, IL-10, IL-1α by dendritic cells was revealed, but there were significant differences in IL-1RA, IL-1α content (p<0.05) with lower level in the group of athletes. Production of IL-17A and IL-7 by dendritic cells in the group of athletes was not detected. In athletes, several proinflammatory cytokines (IFN-α, IL-31, and TNF-β) were secreted by cells in high concentrations, in contrast to the control group. In both groups, dendritic cells did not secrete IL-15, IL-21, IL-22, IL-23, IL-27, and IL-9.

Адрес для переписки:Ханферьян Роман Авакович ФГБУН «Федеральный исследовательский центр питания, биотехнологии и безопасности пищи» 109240, Россия, Москва, Устьинский проезд, 2/14. Тел.: 8 (495) 698-53-45. Е-mail: khanferyan@ion иммунология, 2016. Т. 18, № 5. С. 437-442. doi: 10.15789/1563-0625-2016-5-437-442 © Евстратова В.С. и соавт., 2016 Immunologiya, 2016, Vol. 18, no. 5, pp. 437-442. doi: 10.15789/1563-0625-2016 Federal Research Center of Nutrition and Biotechnology, Moscow, Russian FederationAbstract. The study presents results concerning effects of H 3 /H 4 histamine receptors antagonist (Ciproxifan) upon in vitro chemokine secretion by dendritic cells (DC) and peripheral blood mononuclear cells (PBMC). We have been shown that in vitro inhibition of type H 3 /H 4 histamine receptor affects in different ways production of major chemokines by PBMC and DC, including eotaxin, RANTES, MCP-1, MIP-1α, MIP-1β, GRO-α, and IL-8. E.g., RANTES and GRO-α secretion in DC cultures was reduced, respectively, by 20% and 40%. Meanwhile, MIP-1β secretion was virtually unchanged, whereas MCP-1 secretion exhibited a four-fold increase. In PBMC cultures, the H 3 /H 4 receptor antagonist induced a significant increase in eotaxin, RANTES, MIP-1α, MIP-1β, GRO-α, and IL-8 secretion (p < 0.05).

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