Some important aspects of the principle of the reduction to the pole are examined first. Secondly, a numerical calculation method is presented, followed by two examples, one theoretical, the other practical.
The AFP-synthesizing cells were identified by ultrastructural localization of the antigen in regenerating liver of adult mice after CCl4 poisoning. An indirect immunoperoxidase method with rabbit anti-mouse AFP and peroxidase conjugates of anti-rabbit IgG or their Fab' was used. Good preservation of AFP and tissue structure, and sufficient permeability for the conjugates were obtained after 20' prefixation of small liver specimens in 8% formaldehyde -0.05% glutaraldehyde followed by 16 h fixation in 8% formaldehyde. The intracellular localization of AFP observed in the light microscope in most cases corresponded to its synthesis and secretion. It was found in two cell types, both concentrated mainly in the perinecrotic zones and constituting only a small part of the whole cell population. Most of the AFP-producing cells were normal differentiated hepatocytes without any structural signs of damage. A few smaller cells with active AFP synthesis were present in some animals. By their ultrastructure they resembled the oval cells found during chemical hepatocarcinogenesis in rats.
It is known that good use can be made of calculating the second vertical derivative of gravitational and magnetic fields. The first derivative, or Vertical Gradient, has the same properties, Like the second derivative, it does not depend on the locally linear regional anomaly. Moreover, the gradient is less affected by experimental errors.
The report gives a practical method of calculating the vertical gradient. Its application is identical to that in general use for calculating the second derivative, except that the numerical coefficients are different. The method also allows the establishment of formulae for downward extension of the field and its derivatives.
The AgB10 antigen of bile canaliculi of the mouse hepatocyte was identified using monoclonal antibodies. The Mr value of 116000 for AgB10 was measured by immunoblotting. The tissue localization of AgB10 was studied by light and electron microscopy using the immunoperoxidase technique. AgB10 was predominantly present on the microvillus membrane of bile canaliculi, the brush border of intestinal mucosa and apical surfaces of the epithelial cells in some other organs. A small amount of AgB10 was detected on the basolateral domain of the hepatocytes. AgB10 was specific for hepatocytes and was not found in the other cell types of the liver. In primary hepatocyte culture, AgB10 was localized on the surface of cells during the first 24 h, predominantly at the sites of cell-cell and cell-substratum contacts. After 48 h of culture AgB10 gradually disappeared from contacting cell surfaces and became concentrated only in the reconstituted bile canaliculi.
Results of researches of process of hot rolling of ingots from the experimental aluminum alloy which is economically alloyed by scandium are given. The computer model of process of hot rolling with the use of which the straining, temperature and speed processing modes conditions is calculated and also power parameters of processing is calculated in applied to industrial conditions. It is shown that the use in the model of a certain shape of the ingot faces (Petrov's lock) and indirect rolling action in the edging stand is made it possible to reduce the likelihood of the formation and further development of micro cracks on the edges of rolled metal. The adequacy of results of modeling was confirmed by carrying out pilot-industrial tests when rolling large-size ingots from the experimental alloy and obtaining batches of hot-rolled plates and sheets of various sizes. The tensile test was used to study deformed samples after rolling and samples obtained on five regimes of heat treatment with varying heating temperatures of 300, 350 and 380 oС and holding time in the furnace for 1 and 3 hours. The results of the studies on samples of hot-rolled sheets 10 mm thick showed that, compared with the initial state, the strength characteristics of the metal after heat treatment are reduced by an average of 12-20%, and plastic characteristics increase by 50-65%. In this case, the heat treatment regimes 1 - 3 give a good ratio of the ductility and plastic properties of the metal.
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