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Death receptors (DRs) and the participants of DR mediated signaling are characterized by a large number of mRNA isoforms generated by alternative splicing. Due to their high labor intensity and high cost, conventional methods (RT PCR and RT PCR in real time) are ineffective when the simultaneous detection of a plurality of mRNA isoforms is needed. In this regard, the use of DNA biochips is has prospective appli cations in analyzing the expression of many genes simultaneously. In this paper, we suggest an optimal strat egy of probes selection aimed at detecting the maximum number of mRNA splice variants generated by major participants of DR signaling. The objects of the study were 185 genes that form 1134 mRNA isoforms. As a result, a biochip design was developed that enables the detection of 499 mRNA isoforms (44% of total mRNA splice variants). The proposed strategy combines a high degree of modularity, the use of modern high perfor mance computers, and broad opportunities for setting up the selection criteria in accordance with the objec tives of the study.

Death receptors (DRs) and the participants of DR-mediated signaling are characterized by a large number of mRNA isoforms generated by alternative splicing. Due to their high labor intensity and high cost, conventional methods (RT-PCR and RT-PCR in real time) are ineffective when the simultaneous detection of a plurality of mRNA isoforms is needed. In this regard, the use of DNA biochips is has prospective applications in analyzing the expression of many genes simultaneously. In this paper, we suggest an optimal strategy of probes selection aimed at detecting the maximum number of mRNA splice variants generated by major participants of DR-signaling. The objects of the study were 185 genes that form 1134 mRNA isoforms. As a result, a biochip design was developed that enables the detection of 499 mRNA isoforms (44% of total mRNA splice variants). The proposed strategy combines a high degree of modularity, the use of modern high-performance computers, and broad opportunities for setting up the selection criteria in accordance with the objectives of the study.

No abstract

Preeclampsia is one of the so-called major obstetrical syndromes and a cause of maternal and neonatal morbidity worldwide. In addition, preeclampsia increases the risk of maternal and neonatal cardiovascular disorders and cerebrovascular diseases in the future. During pregnancy, the pregnant mother undergoes significant changes in the functions of various organ systems to accommodate the developing fetus. The onset of preeclampsia can cause dysfunction of multiple organs and systems, including the homeostasis system. Its dysfunction is associated with the most serious thrombohemorrhagic complications (premature detachment of normally situated placenta, thrombosis, embolism, and bleeding). It is important to understand the relationship between the maternal and fetal homeostasis system functioning. The homeostasis system functioning in the fetus and newborn infant has specific characteristics expressed not only in the quantitative composition and the level of activity of its main links, but also in the qualitastive composition, as well as in the functioning mechanisms which differ from those in adults. The above specific characteristics help to achieve an important balance in the homeostasis system functioning. Preeclampsia, associated conditions and complications can disturb the existing balance and homeostasis system functioning in the fetus and newborn infant with the development of thrombohemorrhagic complications. KEYWORDS: homeostasis system, newborn infants, preeclampsia, specific characteristics of functioning, preterm newborn infants, pregnancy. FOR CITATION: Yupatov E.Yu., Kurmanbaev T.E., Mustafin I.G. et al. Hemostasis functioning in neonates: normal condition and condition in preeclampsia (literature review). Russian Journal of Woman and Child Health. 2023;6(2):199–205 (in Russ.). DOI: 10.32364/2618-8430- 2023-6-2-199-205.