Aim. Comprehensive epidemiological assessment of factors affecting the effectiveness of the infertility treatment in medical organizations of St. Petersburg. Materials and Methods. The effectiveness of assisted reproductive technology programs in St. Petersburg reproductive centers was evaluated by analyzing the respective database for infertility treatment in 2013-2017. We retrospectively examined 843 patient records that were submitted to one of the St. Petersburg reproductive centers in 2014-2017 with the following survey and in-depth clinical examination of 129 couples before in vitro fertilisation. Results. Risk factors of infertility included vaginal dysbiosis (OR = 7.5 (95% CI 1.04-54.1)), Trichomonas vaginalis infection (OR= 2.6 (95% For citation:
Relevance. The COVID-19 pandemic has led to significant overloads in the work of health systems in many countries, a shortage of beds and staff, which contributes to a decrease in adherence to measures to prevent and control nosocomial infections, which can significantly worsen the course of viral pneumonia. Aim. To assess the possibility of the formation of hospital strains of multidrugresistant microorganisms in hospitals repurposed to provide medical care to patients with COVID-19. Materials and methods. The study included patients with severe and moderate forms of COVID-19 (ICD codes U07.1, U07.2), who were admitted to two large hospitals repurposed for the treatment of this infection. The data of microbiological studies of the biomaterial associated with the respiratory tract (sputum, bronchoalveolar lavage, tracheal aspirates) obtained from 1101 patients from May to January 2021 were analyzed using a combination of molecular genetic methods (RAPD-PCR, detection of integrons and the carbapenemase gene bla NDM.), and molecular typing of carbapenem-resistant strains of Klebsiella pneumoniae and Acinetobacter baumannii was carried out. Results. It was found that carbapenem resistant gram-negative bacteria predominate in the structure of the nosocomial microbiota of the respiratory tract of patients with COVID-19 in both hospitals. Based on molecular typing made the wide distribution of several genetic lines of integron-positive carbapenem resistant Klebsiella pneumoniae and Acinetobacter baumannii was detected. Conclusions. The COVID-19 pandemic has exacerbated the spread and circulation of bacteria with multiple antibiotic resistance in hospitals. This study has demonstrated the possibility of the formation of hospital strains of nosocomial infections in COVID-19 hospitals, which justifies the need to improve infection control measures in the context of a new coronavirus infection pandemic.
The paper presents optimized methods for PCR and sequence typing of Streptococcus pneumoniae. The serotype composition of pneumococci isolated from children under 5 years of age with infections of the upper respiratory tract was analyzed using optimized methods. Between 2016 and 2021, there was a decrease in the frequency of serotypes included in the pneumococcal 13-valent conjugate vaccine (PCV13) from 94.1 to 25.8%, mainly due to the 6ABCD serogroup and the 19F serotype. The coverage of serotypes circulating in children with PCV15 and PCV20 vaccines was 28.1% and 41.6% in 2021, respectively. During the study period, the number of non-vaccine serogroups 11AD and 15AF, as well as serotypes that are not detected under this capsular PCR typing protocol, increased most significantly.
Assisted reproductive technologies (ART) are one of the most effective ways in fighting infertility, but their effectiveness is influenced by various factors. Our study focuses on examining importance of risk factors underlying ART failure related to altered microbiome pattern in the female reproductive system. The case-control study was based on conducting a laboratory examination of 129 infertile couples applied to the Department of Assisted Reproductive Technologies due to failure of in vitro fertilization (IVF), whereas control group consisted of females with successful progressive pregnancy after ART. Chlamydia, herpes virus and cytomegalovirus were assessed by using PCR and ELISA, whereas culture method was used to detect Trichomonas vaginalis in vaginal swabs and ejaculate applying Trichomonas Modified CPLM Medium (HiMedia, India) followed by microscopy. In addition, the qualitative and quantitative composition of the vaginal biocenosis was studied with multiplex RT-PCR by using Femoflor-16 kit (DNA-technologies, Russia). Serological tests were based on measuring IgG and IgA antibodies against Chlamydia trachomatis in ELISA (diagnostic kits purchased from Vektor Best, Russia; NovaTec, Germany; ImmunoComb, Israel) as well as antibodies against immediate early HSV-1/2 proteins (BioService, Russia) and immediate early HHV-5 proteins (Vector Best, Russia). C. trachomatis and herpesvirus DNA was measured by using PCR diagnostic kits Amplisens (Interlabservice, Russia). It was found that sexually transmitted infection agents were highly prevalent in infertile couples applying to the hospital for IVF. Significant factors for non-pregnancy were vaginal dysmicrobiocenosis (OR = 7.5 (95% CI 1.04—54.1), p = 0.02) and detected T. vaginalis (OR = 2.6 (95% CI 1.12—6.4), p = 0.01). Dysbiosis of the reproductive system, including those occurring due to trichomonas infection is associated with lowered ART effectiveness. It is evident to timely detect urogenital infections and dysbiosis while preparing infertile couples for IVF cycles.
Introduction. The cranial rhythm is represented by the rhythmic movement of the skull bones associated with the cyclic changes in production and pressure of the cerebrospinal fluid. The existing methods of instrumental recording of the quantitative characteristics of the cranial rhythm are either invasive, either contact, or very complex. Objective. To evaluate the EHF-bioradiolocation method as a method of recording of the quantitative characte-ristics of the cranial rhythm. Methods. Twenty volunteers were examined. At the first stage of the study, we determined the optimal conditions for registering the cranial rhythm with the help of the EHF bioradiolocator. At the second stage, the volonteers’ cranial rhythm was registered with the use of an EHF-bioradiolator. Then the volonteers were subjected to osteopathic diagnostics and correction. After that the cranial rhythm was recorded again. Results. For the first time we registered the cranial rhythm with the help of the EHF-bioradiolocator. In most cases, the frequency of the cranial rhythm recorded by the device coincided with the frequency determined by an osteopath. After the osteopathic treatment, the total amplitude of the oscillations increased in all the patients. Assessment of changes in the frequency of the cranial rhythm was difficult due to the presence of several harmonics. Conclusion. With the help of the EHF-bioradiolocator, it is possible to register the quantitative characteristics of the cranial rhythm - frequency and amplitude. The methodology we developed is safe and relatively simple. It is non-contact, and can be used both for diagnostic purposes, and for monitoring and evaluation of the treatment results.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.