The problem of biodiversity conservation and rational use of natural resources remains one of the most pressing problems for countries around the world. To maintain the stability of biological resources a sufficiently developed basis for their reproduction is required, which, in turn requires a preliminary study of the developmental features of individual organisms both in the natural environment and during their introduction into the cultural environment. A special place among the spice plants is occupied by the Lamiaceae Lindl. family. It is rich in medicinal and essential oil plants, which are widely used in family medicine, as well as in folk medicine and food industry, perfumery and cosmetic industries. Such plants include Origanum vulgare and Salvia officinalis. In addition, both plant species are added as phytobiotics to animal feed. The article deals with sterilization of seeds of medicinal plants Origanum vulgare L. and Salvia officinalis L. and the effect of sterilization methods on seed germination. For in vitro cultivation an aseptic, undamaged pure culture was obtained from the original plant material, and the most optimal method for sterilizing the seeds of these medicinal plants was chosen.
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