A new ready-to-use medicinal form -granules containing isoniazid and pyridoxine hydrochloride (0.15 and 0.015 g per gram of granules, respectively) -has been recently developed. An HPLC technique for the qualitative and quantitative analysis of the new preparation is described. The analyses are carried out using a Millihrom-2 chromatograph equipped with a UV detector and a KAKh 4-64-3 column filled with Separon C 18 . The column is eluted with an acetonitrile -0.5% aqueous sodium dihydrophosphate solution (60:40, adjusted with orthophosphoric acid at pH 2.5 -3.0) at a flow rate of 50 ml/min. The detection is carried out at 280 nm. The relative error of determination does not exceed ± 1.1% for isoniazid and ± 4.1% for pyridoxine hydrochloride.
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