We continue analysis of the phenotype of human melanoma cell Mel Ibr subclone obtained previously by treatment of the parental cell line by chicken embryo extract. The present study is focused on detection of markers of epithelial-mesenchymal transition that determine enhanced metastatic and invasive potential of malignant tumors of various locations. Analysis of the expression of E-cadherin and vimentin genes in the subclone and parental cells detected activation of epithelial-mesenchymal transition in the subclone. Immunological markers CD90, CD271, and CD95 were present in the parental population, but were not detected on the subclone cells. In contrast to the parental line, cells of the analyzed subclone retain viability in serum-free medium and formed vessel-like structures characteristic of vasculogenic mimicry.
The operating regimes of a laboratory external combustion engine consisting of a catalytic heater, working cylinder, connection unit with hydroresistance, back pressure unit, and thermochemical recuperator have been studied. An experimental technique that provides an estimate of the power developed by an engine to perform mechanical work has been created. It has been shown that the efficiency can be increased by recu perating the heat of combustion products from the endothermic reaction of the steam that reforms the initial fuel, e.g., propane-butane, into syngas. The effect of the process parameters on an increase in the inner engine efficiency is analyzed. It has been shown that the maximum pressure in the working cylinder has the greatest effect on an increase in the inner efficiency.
Combined technique and setup have been created that combines capabilities of electrochemical methods, as well as high-temperature Raman spectroscopy (RS) at Osipyan Institute of Solid State Physics RAS. In order to study the processes in the electrochemically active zone, a special geometry of samples was developed on basis of optically transparent single crystal membranes of an anionic conductor with a toroidal shape counter-electrode. With the use of this combined technique and special geometry, studies of the kinetics of reduction of nickel in composite SOFC anodes were carried out. The influence of the composition of fuel on Raman spectra obtained from the internal interface in the current load mode was also investigated, and the correlations with the cell voltage were studied. RS is also used to study the structure of single-crystal samples of anionic conductors, including at SOFCs operating temperature. The new combined technique was used to study other components: sealing glasses for SOFCs and optical glasses.
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