The hemorheologic disorders, which are potentially deleterious in a wide range of pathologies, were studied in patients with type-1 and type-2 diabetes mellitus. The significant determinants of blood viscosity, red blood cells (RBC) deformability and RBC reversible aggregation were assessed by ektacytometry and by laser backscattering technique, respectively. For both types of the disease, we observed acceleration of the first phase of RBC aggregation that contrasted with its deceleration at the second phase. The hydrodynamic stability of the aggregates, especially the smallest ones, exceeded the normal values in both cases. The influence of GP IIb/IIIa receptor inhibitor, monafram on RBC aggregation and disaggregation was the same in the cases of patients and normal subjects. The maximal shear induced RBC stretching exceeded the normal one in patients of both groups. The data reveal not only pathological but also compensatory character of hemorheological alterations in patients with type-1 and type-2 diabetes mellitus.
Цель исследования: изучение агрегации эритроцитов, реализующейся в сдвиговом потоке. Материалы и методы. В пробах крови здоровых добровольцев методом регистрации обратного светорассеяния с использованием коаксиально-цилиндрического агрегометра определяли стандартные гемореологические показатели, а также параметры, характеризующие «гистерезис» — разницу в суспензионном состоянии крови при данной скорости сдвига, но разной предыстории течения (его замедлении или ускорении). Результаты. Результаты позволили определить оптимальный показатель «гистерезиса» и охарактеризовать обратимую агрегацию эритроцитов в сдвиговом потоке комплексным образом. Заключение. Предложенный показатель «гистерезиса» позволяет выявлять ослабление и усиление агрегации эритроцитов в норме; однако требуется дополнительное исследование его эффективности при патологии, сопряженной с гемореологическими нарушениями. Aim: to study erythrocytes aggregation realized in a shear flow. Materials and methods. We registered standard hemorheological parameters in healthy volunteers under shear flow conditions using a light scattering concentric cylindrical aggregometer. Also characteristics of «hysteresis» were tested: erythrocytes rates. Results. This comparison revealed «hysteresis» parameters that correlated with low-grade erythrocytes aggregation or with «hyperaggregation» state. Conclusion. The revealed «hysteresis» parameters disclose a balance between erythrocytes aggregation and disaggregation in shear fl ow, but an extension clinical study is required to verify if such analysis would allow for detection of patients with anomalously high erythrocytes aggregation.
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