A rapidly growing development of tissue engineering promotes the increasing interest in the obtainment of various decellularizedtissues and organs. Minimal quality evaluation criteria of obtained tissue engineered constructions have been previously specified. In the discussionpaper the group of authors considers the morphological methods of matrix evaluation applied by various researchers on the model of heart decellularization. The analysis of modern literature and the authors’ own researches have shown that morphological evaluation of decellularization quality has to be complex and should consist of several stages which include both basic and additional evaluation methods.
Aim. To assess the expression of vascular endothelial growth factor A for evaluating the functional state of the endometrium in women suffering from infertility and chronic endometritis.Materials and methods. Endometrium biopsy specimens obtained from 41 patients with uterine factor infertility (experimental group) were examined. A comparison group was composed of 39 women diagnosed with the “male factor” infertility exhibiting no infl ammatory processes of the reproductive organs. An endometrial biopsy was performed in both phases of the menstrual cycle: on days 8–10 of the follicular phase and on days 21–24 of the luteal phase. The expression of vascular endothelial growth factor receptors was evaluated on the basis of immunohistochemical staining of the biopsy specimens using monoclonal antibodies. The results of the immunohistochemical reaction were quantifi ed using the ImageJ software. A quantitative criterion — a stained area coeffi cient (per cent) representing the ratio of the immunohistochemical staining area to that of a biopsy specimen — was proposed for assessing the level of VEGF-A expression.Results. In the chronic endometritis group, the intensity of VEGF-A expression was found to be 1.4 times higher than that in the comparison group during the proliferation phase (p < 0.001). Expression values of the secretion phase were 1.9 times higher than those in the comparison group (p < 0.01).Conclusion. Chronic endometritis is characterized by an overexpression of VEGF-A by various endometrium cellular components. Interpretation of immunohistochemical stains using the method of computer morphometry allows quantitative indicators characterizing the intensity of angiogenesis marker expression in the endometrium during the menstrual cycle to be obtained. The level of VEGF-A expression can be used as an additional marker improving the quality of biopsy diagnostics in patients with infertility.
ОБРАЩЕНИЕ К ЧИТАТЕЛЯМ ОРИГИНАЛЬНЫЕ СТАТЬИХроническая сердечная недостаточность (ХСН) характеризуется высокой частотой распространённо-сти среди населения, неблагоприятным прогнозом и исходом. Об этом свидетельствуют данные значи-тельного числа клинических и эпидемиологических исследований, как в пределах Российской Федера-ции, так и за рубежом [1]. Несмотря на все современ-ные возможности медикаментозных и хирургических STRUCTURE OF MORPHOLOGICAL CHANGES OF MYOCARDIUM IN NON-CORONARY ORIGIN SYSTOLIC DYSFUNCTION OF THE LEFT VENTRICLEBakhchoyan m. r. We got following results: definite myocarditis -1,39%, possible myocarditis -9,71%, myocarditis cardiosclerosis -6,94%, non-inflammatory cardiomyopathy -38,9%, amyloidosis -1,39%. In 37,5% cases myocardial specimens were of common histological structure, without signs of current inflammation and myocyte necrosis; 4,17% of specimens were non-informative due to lack of amount for assessment. Conclusion.The data obtained in the study makes it possible to define nosological structure of severe systolic dysfunction of the LV of non-coronary origin in Krasnodarsky region. Taken the absence in russian Federation of any large scale epidemiological studies of the problem, it is necessary to elaborate database and to implement it into routine practice of cardiologists. Such data might improve diagnostics, etiotropic and pathogenetic therapy, and improve outcomes of the diseases group.Russ J Cardiol 2016, 1 (129): 47-50 http://dx
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