Аннотация. В ходе исследования выявлены ДНК-маркеры к местным расам L. maculans и установлено, что в популяции отобранного гриба присутствует последовательность AvrLm4-7, которая специфично распознается генами устойчивости Rlm4 и Rlm7. Выявлен SCAR-маркер BN204, позволяющий идентифицировать гомозиготные и гетеро-SCAR-маркер BN204, позволяющий идентифицировать гомозиготные и гетеро-маркер BN204, позволяющий идентифицировать гомозиготные и гетеро-BN204, позволяющий идентифицировать гомозиготные и гетеро-204, позволяющий идентифицировать гомозиготные и гетерозиготные растения, несущие ген Rlm4. С использованием ДНК-маркера BN204 была проанализирована рабочая коллекция, включающая 22 сорта и 39 сортообразцов рапса. Результаты ПЦР позволили установить, что количество индивидуальных растений с геном устойчивости Rlm4 было выше, чем количество устойчивых форм, охарактеризованных путем заражения листовых эксплантов расами патогена, несущими последовательность AvrLm4-7. Предполагается, что данные индивидуальные растения содержат ген Rlm4, однако у них отсутствует ген Rlm7. Ключевые слова: маркер-сопутствующая селекция, рапс, гены Avr, гены Rlm, устойчивость к фомозу Для цитирования: Роль генов RLM и AVRLM в реализации специфической устойчивости к фомозу у рапса / В. А. Лемеш [и др.] // Докл.
We evaluated the rate of polymorphism of doubled haploid lines of hexaploid triticale obtained by the method of anther culture based on hybrids of spring and winter types. Using 7 SSR markers for the loci on the chromosomes A- (Xgwm186, Xgwm291, Xgwm595) and B- (Xgwm371, Xgwm540, Xgwm554, Xgwm234), polymorphism of 38 doubled haploid lines of hexaploid triticale was studied. Interlinear polymorphism along six microsatellite loci except the Xgwm554 locus, which is not polymorphic in the studied doubled haploid lines, was revealed. The highest polymorphism was observed for the Xgwm186, Xgwm291 and Xgwm595 loci. The cluster analysis showed that all studied lines were divided into three main groups. The origin of the lines did not affect the distribution in groups. This confirms the influence of in vitro culture somaclonal variation. Eight lines of doubled haploids, which are heterozygous for one of the studied microsatellite loci, were identified. We showed the possibility of using SSR markers to assess interlinear polymorphism and the homozygosity rate in the triticale doubled haploid lines obtained by the method of induced androgenesis in vitro.
Based on bioinformatics and statistical analysis of GEO-projects on the determination of the genome-wide profile of human DNA methylation, a list of CpG dinucleotides with high predictive potential was formed to design models allowing to predict human age by sperm samples. The methylation level was determined for 467 males from the Republic of Belarus. The correlation coefficients R were calculated, and mathematical models for determining the age of an individual were constructed. The average value of the accuracy of age prediction by sperm samples using 5 CpG-dinucleotides was 3 years. The results obtained will be used as a basis for the development of calculators for predicting the age of an individual based on the traces of biological character for forensic experts.
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