The effects of α-tocopherol with shortened to 6 carbon atoms side chain ), α-tocopherol succinate (α-Ts) and quinonimine 2,6-dichlorophenolindophenol (DCPIP) on DT-diaphorase activity and viability of rat thymocytes, splenocytes and hepatocytes were investigated. It was shown that the lowest basal activity of the enzyme is inherent in splenocytes. In comparison to splenocytes, DT-diaphorase activity was 1.4 and 5 times higher in thymocytes and hepatocytes, respectively. It was found that the sensitivity of cells to the cytotoxic effect of DCPIP was inversely proportional to the basal level of DT-diaphorase activity and accompanied by its activation with subsequent inhibition at non-toxic and toxic concentrations, respectively. hepatocytes were least sensitive to the cytotoxic effect of α-Toc-C 6 . In thymocytes and splenocytes α-Toc-C 6 exerts inhibitory effects on DT-diaphorase, whereas in hepatocytes an increased activity of the enzyme was observed, which probably caused their high survival rate. simultaneous induction of cytochrome P450 enzyme expression by α-Toc-C 6 in hepatocytes is also possible. Cytotoxic effect of α-Ts does not depend on the basal level of DT-diaphorase activity in cells, is not accompanied by its induction and it is most likely determined by the non-specific esterase activity. k e y w o r d s: α-tocopherol with shortened to 6 carbon atoms side chain (α-Toc-C 6 ), α-tocopherol succinate (α-Ts), quinonimines, thymocytes, splenocytes, hepatocytes, DT-diaphorase.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.