A micromethod for the determination of metabolites in muscleA micromethod is described for the analysis of glycolytic intermediates and high energy phosphates in muscle tissue. The tissue is frozen, sliced to 10-20 thickness in a cryostat, and the slices submitted to ultrasonic disruption. The metabolites are determined fluorimetrically. The composition of the test mixture is given for the following substrates: G-6-P, G-l-P, F-6-P, FDP, DAP, G, M, L, PY, ATP, ADP, AMP, CP and glucose. The values for ATP and CP obtained by this method are only 11 and 19% higher, respectively, than those hitherto obtained by other methods. The values for the glycolytic intermediates are, however, very much lower than those measured previously. The L/P quotient, calculated from the concentrations of L and PY, is about 12; it is thus close to that for other organs and fluids. The low value for glycolytic intermediates and the L/P quotient show that the concentrations determined by the above micromethod approach [ those obtaining in vivo.
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