Iron chelation therapy is commonly used in patients with myelodysplastic syndrome (MDS), to prevent majorcomplications of iron overload. Besides effects on maintaining control of iron stores and preventing iron-induced cardiac disease, the impact of chelation therapy on overall survival and leukemia-free survival in MDS has been documented, but not well understood. Since MDS bone marrow cells are known to activate DNA damage response (DDR) signaling and iron chelators target cancer cells through multiple stress-response mechanisms (endoplasmic reticulum (ER) stress, autophagy), we hypothesized that iron chelation could reinforce DDR signaling and could thus support tumor-suppressing role of DDR. Also nucleotide deficiency was shown to contribute to DDR, and iron chelation is known to inhibit ribonucleotide reductase (RR), an iron-dependent enzyme, which supplies cells with deoxyribonucleotides (dNTPs). Here, we tested the effects of lysosomotropic iron chelator deferoxamine mesylate (DFO) in a preleukemia mouse model, wherein epigenetic oncogene-induced leukemogenesis is preceded with a long-lasting preleukemia stage (Takacova S, et al. Cancer Cell. 2012;21(4):517-31.). Preleukemic, aberrantly proliferating myeloid cells in this model activate a replication checkpoint and ATR-Chk1-mediated DDR (consistent with oncogene-induced replication stress) and attain hallmarks of senescence (with a long latency), resulting in the inhibition of leukemia progression. A group of 10 preleukemia mice and a group of 10 control mice aged 7 month were treated twice daily with DFO doses adjusted to 88,8 mg/kg (i.p. injection) in order to mimic serum concentrations of the drug achieved in patients. After 6 weeks of chelator administration, the treatment lead to the activation of Chk1(S345) in the bone marrow (BM) of control mice, but did not result in accumulation of γH2AX, a marker of DNA damage, in BM of these mice. In contrast, in preleukemia mice, with already activated threshold of ATR-Chk1 signaling (marker of ongoing oncogene-induced replication stress), Chk1(S345) remained unchanged after DFO treatment. However, we observed significant accumulation of γH2AX foci in oncogene-positive BM cells. These data suggested that iron removal may induce Chk1 activation in vivo, and, in addition, may reinforce activation of DDR in preleukemia cells perhaps due to synthetic effect of iron chelation with oncogene activation resulting in increased levels in DDR signaling (assessment of oxidative DNA damage (8-oxoguanine staining) is ongoing). Next, we analyzed whether iron chelation in both groups of mice influences DNA replication, in which the limiting step is the availability of dNTPs. The RR activity was significantly decreased in the BM of both groups of DFO-treated mice, however, with no impact on the concentration of BM dNTPs; in fact, dNTPs have accumulated in BM of these mice. We revealed that this was a consequence of the activation of S-phase checkpoint in control mice, and of a decrease of actively replicating myeloid cells and activation of G2/M checkpoint in preleukemia mice. Cellular iron depletion was shown to activate p38MAPK pathway (Yu Y, Richardson DR. J Biol Chem. 2011;286(17):15413-27.). p38MAPK pathway, and its component MK2, establishes intra-S-phase cell cycle checkpoint and activates G2/M checkpoint (as a part of DDR, in parallel to Chk1 activation (Reinhardt HC, et al. Curr Opin Cell Biol. 2009;21:245-55.)). Indeed, our preliminary result revealed phosphorylated MK2 specifically in preleukemia mouse BM treated with DFO. Since we did not detect increased apoptosis in BM of DFO treated mice, and because p38MAPK pathway is involved in the activation of ER stress and autophagy, we tested whether markers of ER stress and autophagy are detectable in the mice upon DFO treatment. MyD116 (marker of recovery from ER stress) and LC3-II (marker of autophagy), were specifically induced in preleukemia cells upon DFO treatment. Collectively, these data demonstrate that preleukemia cells exposed to DFO activate distinct but functionally overlapping signaling pathways, resulting in reinforced DDR. Whether this mechanism could increase a barrier against leukemia transformation of chelated MDS patients remains to be investigated. Authorship: LRK and ZS: equal credit as first authors. Acknowledgment: Supported by the Czech Science Foundation (P301/12/1503) and by IGA_LF_2015_015. Disclosures No relevant conflicts of interest to declare.
Class III receptor tyrosine kinases (RTK), which include c-fms, c-kit, FMS-like tyrosine kinase receptor-3(FLT3) and platelet-derived growth factor receptor (PDGFR) α/β are expressed on acute myelogenous leukemia (AML) cells from the majority of patients and stimulate survival and proliferation of leukemic blasts. FLT3 activation cooperates, for instance, with oncogenic mixed lineage leukemia (MLL) fusion proteins in MLL-induced transformation. The most common FLT3 activation mutation, internal tandem duplication (ITD), is the most frequently observed molecular defect in AML, and it is associated with early relapses and poor prognosis. FLT3-ITD leads to constitutive, ligand-independent activation of the kinase; this results in FLT3 autophosphorylation and induction of several downstream signaling cascades including Ras/MAPK kinase (MEK)/extracellular signal-regulated kinase (ERK) and STAT5 pathways. FLT3 as well as other class III RTK have been widely accepted as suitable drug targets. Several potent inhibitors have been developed, and some of them, such as quizartinib or crenolanib, have demonstrated promising clinical outcomes. However, resistance to these inhibitors remains a significant clinical problem; therefore, development of novel inhibitors is needed. Also Src family tyrosine kinases (SFK) have been proven as therapeutic targets in multiple cancers including leukemia. Here, we developed and tested a novel series of compounds which revealed dual inhibitory activities against class III RTK and SFK, and tested them in vitro against FLT3- and PDGFRα-mutated leukemic cells and in vivo against FLT3-ITD-positive AML. First, we tested kinase selectivity of the novel compounds. Kinase-inhibitory properties were screened at single concentration of 10 nM in biochemical phosphorylation assays against 300 kinases. The compounds revealed strong and specific inhibitory activity especially against class III RTK and SFK. Then, we have used multiple cell lines harboring various oncogenic kinases to test in vitro growth inhibition potential of the compounds. The most potent newly synthesized inhibitor, designated 3922, showed EC50 values at low nanomolar concentrations against FLT3-ITD-positive cell line MV4-11 and FIP1L1-PDGFRα-positive EOL-1 cells. We also used primary cells derived from mouse bone marrow bearing inducible fusion oncogene MLL-ENL-ER (MEER) adapted to growth in cell culture (Takacova et al, 2012, Cancer Cell 21:517). Prior to drug testing, the MEER cells were grown in cell culture media to induce cytokine addiction either to stem cell factor (SCF) or to ligand of FLT3 (FLT3L). The efficacy of 3922 was 5 times more potent against FLT3L-addicted MEER cells than against SCF-addicted cells suggesting that FLT3 is the main target of 3922. We then compared the effect of 3922 on inhibition of FLT3 phosphorylation (pFLT3-Tyr589/591) with quizartinib (Zarrinkar et al, 2009, Blood 114:2984). Both compounds showed to be very efficient inhibitors of FLT3 phosphorylation at nanomolar concentrations, however, 3922 inhibitory effect had longer durability after drug withdrawal when compared with quizartinib. Finally, we determined the activity of 3922 in vivo. A single-dose of 10 mg/kg of 3922 or quizartinib was administered to the mice with subcutaneously implanted MV4-11 xenograft. Quantitation of pFLT3 revealed that the RTK was inhibited by 95% even after 2 hours administration of 3922 and this inhibition sustained 24 hours, in contrast to elevated pFLT3 24 hours after quizartinib administration (Zarrinkar et al, 2009; Gunawardane et al, 2013, Mol Cancer Ther 12:438). After 2 hours, the pERK1/2 levels were reduced by both inhibitors, however, returned to phosphorylation levels comparable to vehicle treated control in 24 hours after administration. The inhibitory effect was more pronounced on phosphorylation of STAT5. Inhibitor 3922 reduced the pSTAT5 level by more than 95% after 24 hours, slightly more effectively than quizartinib. Induction of apoptosis was assessed by PARP cleavage. Cleaved PARP was significantly elevated by 3922 even after 2 hours of treatment, with a pattern similar to the PARP cleavage induced by quizartinib (Gunawardane et al, 2013). In conclusion, we have developed a novel highly potent tyrosine kinase inhibitor effective against AML in vitro and in vivo. Acknowledgment: Supported by NV15-28951A from Ministry of Health, Czech Republic. Disclosures No relevant conflicts of interest to declare.
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