One of the main targets of high‐value oils is focused on polyunsaturated fatty acids (PUFAs) that have been incorporated into several products ranging through feeds, foods, cosmetics and pharmaceuticals. Biotechnological strategies based on solid state fermentation have been used to enrich cereals with PUFA as a major food source. Under appropriate conditions, lower filamentous fungi were able to transform numerous cereal substrates to products containing various n‐6 and n‐3 PUFAs.
Effects of various cultural conditions on biomass, lipid and Gamma-linolenic acid (GLA) production were investigated in the oleaginous fungus Cunninghamella blakesleeana-JSK2 isolated from soil. The GLA production was influenced by various factors such as growth condition (static and shaken), incubation time, pH, temperature, carbon and nitrogen sources. The results indicated that optimum GLA production (21 %) was obtained when the fungus was grown under shaken condition at 120 rpm for 6 days with optimum pH and temperature of 6 and 28°C ,respectively. Glucose and potassium nitrate enhanced the GLA production. Urea and sucrose were poor substances for biomass, lipid and GLA production.
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