Long noncoding RNAs (lncRNAs) play crucial roles in regulating a variety of biological processes in lung adenocarcinoma (LUAD). In our study, we mainly explored the functional roles of a novel lncRNA long intergenic non-protein coding RNA 1426 (LINC01426) in LUAD. We applied bioinformatics analysis to find the expression of LINC01426 was upregulated in LUAD tissue. Functionally, silencing of LINC01426 obviously suppressed the proliferation, migration, epithelial–mesenchymal transition (EMT), and stemness of LUAD cells. Then, we observed that LINC01426 functioned through the hedgehog pathway in LUAD. The effect of LINC01426 knockdown could be fully reversed by adding hedgehog pathway activator SAG. In addition, we proved that LINC01426 could not affect SHH transcription and its mRNA level. Pull-down sliver staining and RIP assay revealed that LINC01426 could interact with USP22. Ubiquitination assays manifested that LINC01426 and USP22 modulated SHH ubiquitination levels. Rescue assays verified that SHH overexpression rescued the cell growth, migration, and stemness suppressed by LINC01426 silencing. In conclusion, LINC01426 promotes LUAD progression by recruiting USP22 to stabilize SHH protein and thus activate the hedgehog pathway.

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Among numerous indoor localization systems proposed during the past decades, WiFi fingerprint-based localization has been one of the most attractive solutions, which is known to be free of extra infrastructure and specialized hardware. However, current WiFi fingerprinting suffers from a pivotal problem of RSS fluctuations caused by unpredictable environmental dynamics. The RSS variations lead to severe spatial ambiguity and temporal instability in RSS fingerprinting, both impairing the location accuracy. To overcome such drawbacks, we propose fingerprint spatial gradient (FSG), a more stable and distinctive form than RSS fingerprints, which exploits the spatial relationships among the RSS fingerprints of multiple neighbouring locations. As a spatially relative form, FSG is more resistant to RSS uncertainties. Based on the concept of FSG, we design novel algorithms to construct FSG on top of a general RSS fingerprint database and then propose effective FSG matching methods for location estimation. Unlike previous works, the resulting system, named ViVi, yields performance gain without the pains of introducing extra information or additional service restrictions or assuming impractical RSS models. Extensive experiments in different buildings demonstrate that ViVi achieves great performance, outperforming the best among four comparative start-of-the-art approaches by 29% in mean accuracy and 19% in 95th percentile accuracy and outweighing the worst one by 39% and 24% respectively. We envision FSG as a promising supplement and alternative to existing RSS fingerprinting for future WiFi localization.

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This paper constructed the overall framework of a real-time virtual campus, and achieved the modeling, environment setting and routing path generating with Multigen software. The puzzles in geometric modeling and virtual scene construction were also discussed in this paper, while the real-time routing was finally achieved in the virtual campus system. The content above would be helpful for the further projects about virtual campus.

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A sound from long distance may arrive different sensors at different moment. Existing field Anti-Sniper systems always need 4 or more sound sensors to locate sound source. This paper gave a method to make some assumptions and estimate the orientation of the sound source with fewer sensors. The following simulation gave the calculation contrast, which showed that the method in paper could narrow the scope of sound source target effectively, and promote the practical solution of Anti-Sniper system in battlefield.