The reconstruction of the three-dimensional (3D) morphology of polymeric microsphere layers based on confocal Raman microscopy was studied. Refraction of the Raman laser beam at the curved surface of the spheres broadens the focus volume inside the sphere. Compared to planar layers, the focus gets trapped inside the spheres such that the measured depth profiles are shifted and broadened. Additionally, the Raman signal of the underlying substrate is already observed for nominal focus positions above the microsphere layer. The results are successfully modeled with ray-optical simulations that allow for a clear understanding of the relevant mechanisms that lead to the generation of the Raman signals in the complex three-dimensional structures.
Nanocrystals of alkaline niobates are currently being discussed for various applications because of their diverse and remarkable properties. Although the growth of bulk niobate crystals is well established, little is known about respective nanocrystals and the optical properties of niobates below 100 nm. A systematic view of the hydrothermal synthesis of potassium niobate with respect to the precursor species reveals the sensitive dependence of the resulting crystalline phases and sizes on the educt modifications. With a variation of stoichiometry of the procedure, the product modification and crystallite size can be changed. By means of second harmonic generation, nanocrystalline potassium niobate offers the possibility for use as an optical marker in high resolution nonlinear microscopy. Redispersed particles show a significant second harmonic generation signal throughout the visible spectral range.
Encapsulation of iron oxide and gold nanoparticles into the bilayer structure of transferrin-modified niosomes enables greatly enhanced and contamination-free SERS-signals in vitro as well as a dual-targeting functionality towards cancer cells.
BackgroundIn vitro and in vivo biosensing through surface-enhanced Raman scattering often suffer from signal contamination diminishing both the limit of detection and quantification. However, overcoming the lack of specificity requires excessive nanoparticle concentrations, which may lead to adverse side effects if applied to patients. ResultsWe propose encapsulation of iron oxide (FexOy) and gold (Au) nanoparticles (NPs) into the bilayer structure of transferrin-modified niosomes. This approach enables achieving greatly enhanced and contamination-free SERS-signals in vitro as well as a dual-targeting functionality towards MCF-7 breast cancer cells. An in-depth characterization of FexOyNPs- and AuNPs-loaded niosomes (AuNPs/FexOyNPs/NIO) after magnetic downstream processing reveals defined hybrid niosome structures, which show a long-term SERS-signal stability in various media such as MCF-7 cell culture medium. In vitro 2D-SERS imaging unveil a successful incorporation of a non-toxic dose of hybrid NPs into MCF-7 cells, which leads to strong and almost contamination-free SERS-signals. The measured signal-to-noise ratio of the in vitro signal exceeds the values required by DIN 32645 for the successful validation of a detection method. ConclusionsThe hybrid niosomes can be considered a promising and efficient agent for the establishment and commercialization of a highly sensitive detection kit for monitoring cancerous tissue.
This paper introduces an approach that enables highly adjustable surface adsorption of single plasmonic nanostructures using polar surface arrays. The plasmonic nanostructures are made from DNA origami and functionalized with gold nanoparticles for surface-enhanced spectroscopic techniques. To ensure that the contribution of individual nanostructures to the measured signal can be detected without any interference from the surrounding structures, we aimed to control the distance and set a minimum gap between the nanostructures on the substrate surface. We describe the fabrication process of the polar surface array based on electron beam lithography, followed by functionalization. Our results indicate that the concentration of DNA origami structures and the duration of the incubation primarily affect the number of adsorbed nanostructures. Density functional theory simulation explains the selective adsorption of plasmonic nanostructures due to the substrate surface properties. The spatial arrangement of nanostructures allows for the reliable identification of the Raman signal’s location, while a falsified identification resulting from agglomeration is prevented.
Purpose The purpose of this paper is to improve the reliability of the force measurement system by determining the reliable test range of dynamometer. Design/methodology/approach Based on the principle of leverage and moment balance, a general force distribution model is applicable in where the test point is located either inside or outside the support region of four three-component force links of dynamometer is established. After corroborating the correctness of the model through verification experiments, the boundary conditions that each three-component force link should satisfy are analyzed by considering the characteristic of the dynamometer components comprehensively. Furthermore, the reliable test range of dynamometer is determined, followed by a calibration experiment to verify its rationality. Findings The relationships between the reliable test range and the tested force, the bolt pre-tightening force and the bearing capacity of quartz wafers are clarified. Further, the experimental calibration results show that when the test point is within the reliable test range, the three-directional output voltage of dynamometer has excellent linearity and repeatability. The nonlinearity and repeatability in X-, Y- and Z-directions are all less than 1.1%. Originality/value A general mathematical model of force distribution of four three-component force links is constructed, which provides a theoretical basic for the mechanical analysis of multi-sensors’ dynamometer. Comprehensively considering the performance of dynamometer components, the value of measured force and the pre-tightening force, the simultaneous equations of reliable test range are deduced, which limits the boundary of allowable test position of piezoelectric dynamometer.
In confocal Raman microscopy, depth profiling is a key application that enables analysis of the structural and chemical composition and size of three-dimensional (3D) transparent objects. However, the precise interpretation of a probed sample's Raman depth profile measurement can be significantly affected by both its size and surrounding objects. This study provides a more comprehensive understanding of the observed optical effects at the interface between polymer spheres and different substrates. Ray- and wave-optical simulations support our results. We derive a correction factor that, depending on the instrumental configuration, allows us to determine the nominal dimensions of the scanned objects more accurately from Raman depth profiles. Our studies support the need for careful consideration when employing depth profiling in confocal Raman microscopy for nondestructive, quantitative tomography of 3D objects.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
334 Leonard St
Brooklyn, NY 11211
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.