Much work has been done on the isolation, purification, and characterization of the RNA-directed RNA polymerase (EC 2.7.7.48) of cucumber mosaic virus (CMV)-infected cucumbers. Uninfected plants were reported to have no such enzyme, but we recently detected low levels of the activity in cucumber. Since tobacco and cowpea contain such an enzyme that is variably increased in amount by various virus (as well as viroid) infections, we assumed that this would also be the case upon CMV infection of cucumber. However, further purification and characterization of the RNA-directed RNA polymerases from healthy and from infected cucumber suggests that these are different enzymes. The presumed CMV replicase was obtained pure and consists of a major polypeptide of Mr 100,000 and minor components of Mr 110,000 and about 10,000. The Km is 5 pM ([3H]GTP) when tobacco mosaic virus RNA is used as template.The role of plant RNA-directed RNA polymerases (EC 2.7.7.48) in the replication ofRNA viruses remains uncertain. The RNA of cowpea mosaic virus, a virus resembling picornaviruses (1), appears to become replicated without participation of the cowpea RNA-directed RNA polymerase, although the amount of the enzyme is greatly increased by that virus infection (2-4). Several studies of viral replication complexes-particulate aggregates containing several proteins, membranous structures, and RNA-have given conflicting results in terms of containing the host enzyme and/or one or several viral gene products (5)(6)(7). In barley infected with brome mosaic virus (BMV), evidence for a virus-specific component in viral RNA replication was reported (5, 8). However, in tobacco no evidence was found for any role of a protein encoded by the infecting tobacco mosaic virus (TMV) in viral RNA replication (9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20).The tobacco enzyme, as well as those of other plants, have molecular weights of 130,000-140,000. In contrast, the presumed viral RNA replicases have approximate molecular weights of 180,000 plus possibly 130,000 (TMV and turnip yellow mosaic virus) or 110,000 and possibly 70,000 [BMV and cucumber mosaic virus (CMV)]. Yet the molecular weight of the largest gene product of tobacco necrosis virus is only about 65,000. The need for both host and viral components for viral RNA replication is quite likely. This has been observed to be the case with the RNA bacteriophages and picornaviruses. Our present concern has been with cucumber, a plant reported to lack RNA-directed RNA polymerase, and the replication of CMV in this plant. We have found small amounts ofRNA-directed RNA polymerase in cucumber, partially characterized it, and compared it with the enzyme, produced in greater amount and obtained in pure form, from CMV-infected cucumber. It appears that the presumed CMV replicase has a molecular weight of about 100,000 and 110,000, whereas that of the cucumber enzyme is about 140,000. The earlier report that the Mr 100,000 enzyme differs in peptide pattern from the viral gene product corresponding ...
Polynucleotide phosphorylase (polyribonucleotide:orthophosphate nucleotidyltransferase, EC 2.7.7.8) activity has been found in many prokaryotes and studied in detail since 1955. Such enzymes have been detected also in plants. We now describe the purification of polynucleotide phosphorylase from cucumber cotyledons and leaves. This enzyme is a complex of three subunits, possibly not identical, of about Mr 50,000. Its enzymatic properties are similar to those of the tobacco enzyme. Unlike the prokaryotic enzymes, the plant enzyme shows activity in the absence of primer but is to various extents stimulated by various ribopolynucleotides or RNAs. RNA-dependent RNA polymerase, not previously shown to exist in non-virus-infected cucumber, has been found to be present at a low level and was separated from the much greater amount of polynucleotide phosphorylase, although some of the physical properties of the two enzymes are rather similar.In the course of a continuing study of the RNA-dependent RNA polymerase of cucumber cotyledons and leaves, it became evident that these plants, be they virus-infected or not, contained much of another enzyme, polynucleotide phosphorylase (polyribonucleotide:orthophosphate nucleotidyltransferase, EC 2.7.7.8). Polynucleotide phosphorylase was first discovered and purified from Lactobacillus and has since been isolated from many other aerobic and anaerobic bacteria (1)(2)(3)(4). It has proved to be a most useful tool in many molecular biological studies requiring polynucleotides of known composition, with one of the first important applications serving in the establishment of the genetic code. However, the role of these enzymes in prokaryotes has not yet been identified. It has been suggested that they could serve as alternate means to nucleases for the degradation of unwanted RNA, but only in systems in which the inorganic phosphate concentration is sufficiently high to reverse the reaction.Polynucleotide phosphorylase was detected, characterized, and partially purified from tobacco by Brishammar and Juntti (5). Its presence in animal cells is scanty and dubious (5-7). Del'vig and co-workers (8, 9) have studied the particular association of such enzymatic activity with polysomes.We now have isolated an almost pure enzyme of properties similar to those of tobacco (5)
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