Methacrylated gelatin films with in situ-generated TiO 2 nanoparticles containing varying weight percentages of gelatin (0 %, 0.5 %, 1 %, 2 % and 4 %) were successfully prepared as novel biomaterials. 1 H-NMR spectroscopy confirmed their methacrylation with a 79 % degree of substitution. TiO 2 nanoparticles were uniformly distributed in the films with the average particle size increasing from 85 to 130 nm in proportion to an increase in TiO 2 concentration from 0.5 to 4 wt%. The water absorption of various gelatin methacrylamide/TiO 2 films was in the range of 471-758 %, which was enough to prevent wound beds from exudates accumulation. And in vitro degradation test in PBS showed that the three-dimensional structure of all samples basically remained unchanged although more than or nearly half the mass of specimens decreased after 4 weeks' degradation, and the pH levels of all sample solutions were maintained in an adequate range of 6.5-7.4 for cell and tissue growth during the whole process. The antibacterial activities of the films against E. coli and S. aureus were measured via a shake flask test and demonstrated good performance after the importation of TiO 2 nanoparticles. Cytotoxicity testing revealed that all films had no cytotoxicity and showed favorable adherence in the presence of L929 cells. The results suggest that hybrid hydrogel films hold potential for antibacterial wound dressing and tissue engineering scaffold applications.

GSP/gelatin composite nanofiber membranes containing silver nanoparticles were successfully fabricated as a novel biomaterial by electrospinning. The silver nanoparticles (AgNPs) were synthesized with the grape seed polyphenols (GSP) as reducing agent in aqueous solution of gelatin, and then the GSP/gelatin/AgNPs mixed solution was electrospun into nanofibers at 55 o C. The scanning electron microscopy (SEM) confirmed that the composite fibers were uniform and the average fiber diameter ranged between 150 nm and 230 nm with an increase in applied potentials from 14 kV to 22 kV. And the transmission electron microscopy (TEM) showed that silver nanoparticles distributed individually in the fibers with the average particle size of about 11 nm. Furthermore, the ultraviolet visible spectrophotometer (UV-vis spectroscopy) test demonstrated that all of Ag + converted to Ag 0 when the concentration of gelatin was 24 wt% and the mass ratio of GSP to AgNO 3 was about 5:2. The antibacterial activities of the fiber membranes against E.coli and S.aureus were measured via a shake flank test and demonstrated good performance after the importation of silver nanopaticles. Cytotoxicity testing also revealed that fiber membranes contained silver nanoparticles had no cyto-toxic. All the results indicated that the GSP was effective for the formation and stabilization of silver nanoparticles in composite nanofibers mats which had the potential for applications in antimicrobial tissue engineering and wound dressing.