A Perilla frutescens (L.) Britt.(Lamiaceae) é uma planta famosa usada na medicina tradicional chinesa para o tratamento de diversas doenças. A fim de avaliar a qualidade da P. frutescens de forma simples, rápida e precisa foi desenvolvido um método de cromatografia líquida de alta performance (CLAE) para caracterização dos dois ácidos triterpenos bioativos: o ácido oleanólico (OA) e o ácido ursólico (UA). Foi usada uma coluna Kromasil 100 C18 RP, com metanol e solução de ácido fosfórico (H 3 PO 4 ) como fase móvel e detecção a 210 nm. O método foi preciso, com um desvio-padrão relativo para esses dois componentes variando entre 0,3-0,6% (análises feitas no mesmo dia) e 0,6-1,2% (análises em dias diferentes). As quantidades de OA e UA na P. frutescens foram determinados por recuperação, variando de 96,7 a 102,0%. Os teores desses dois fitoquímicos encontrados em diferentes partes da P. frutescens cultivada em cinco locais da China, foram determinados para verificar a eficácia do método.Perilla frutescens (L.) Britt.(Lamiaceae), a famous traditional Chinese medicine, has been used for the treatment of various diseases. To evaluate the quality of P. frutescens, a simple, rapid and accurate high-performance liquid chromatography (HPLC) method was developed for the assessment of two bioactive triterpene acids: oleanolic acid (OA) and ursolic acid (UA). The HPLC system used an Kromasil 100 C 18 RP column with methanol and aqueous H 3 PO 4 as the mobile phase and detection at 210 nm. The method was precise with relative standard deviations for these two constituents that ranged between 0.3-0.6 % (intraday) and 0.6-1.2 % (interday). The contents of the OA and UA in P. frutescens were determined with recoveries ranging from 96.7 to 102.0%. The content of these two phytochemicals in different parts of P. frutescens growing at five different locations of China were determined to establish the effectiveness of the method.Keywords: Perilla frutescens, Lamiaceae, HPLC, oleanolic acid, ursolic acid IntroductionPerilla frutescens (L.) Britt. (Lamiaceae) is known as the wild/weedy form, which is found in China, Korea and Japan. It is used as a spicy vegetable and as an oil crop. 1 P. frutescens, which is a traditional Chinese medicinal herb, has been used in China for centuries to treat various diseases including depression, anxiety, tumor, cough, bacterial and fungal infections, allergy, intoxication and some intestinal disorders.2-5 It has been reported that triterpenoid compounds isolated from the leaves of P. frutescens, the two main bioactive constituents were identified spectroscopically and chemically as oleanolic acid (OA) and ursolic acid (UA) (Figure 1). 6,7 OA and UA were both reported to have the anti-inflammatory activity. 8,9 Furthermore, OA possesses anti-ulcer 10 and hepatoprotective activities, 11 and UA exerts its anti-tumor action through enhancing the production of both nitric oxide and tumor necrosis factor-.12 Needless to say, a sufficiently high content of the bioactive phytochemicals is crucial for therap...
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