Osteosarcoma (OS) is the most common primary malignant bone tumour among adolescents and young adults; however, its molecular pathogenesis has not been completely elucidated. Ubiquitin-specific protease 7 (USP7), a member of the deubiquitinating enzyme family, plays a role in the malignancy process of various cancer types by targeting the key oncoprotein; however, its biological function and mechanism in OS have not been elucidated. The present study demonstrated that USP7 expression in OS tumour tissues was markedly higher than that in the paired surrounding tissues, and high USP7 expression was positively correlated with the TNM stage and metastasis in patients with OS. Next, biological function assays demonstrated that USP7 knockdown markedly inhibited OS cell migration and invasion, whereas USP7 overexpression enhanced it. Notably, USP7 can directly bind with β-catenin to activate the Wnt/β-catenin signalling pathway and induce epithelial-mesenchymal transition (EMT) of OS cells. Overall, USP7 overexpression could promote OS cell metastasis by activating the Wnt/β-catenin signalling pathway by inducing EMT, suggesting that USP7 is a potential therapeutic target for OS.
A rational design of a Pd catalyst with highly dispersed Pd nanoclusters on an Al doped ceria-based oxide for low temperature selective catalytic reduction of NO by hydrogen with excess O was achieved. The supported Pd nanocluster shows a high hydrogen spillover ability and a NO conversion of >84% within 100-300 °C.
Dynamic fault tree is often used to analyze system reliability. The Markov model is a commonly used method, which can accurately reflect the relationship between the state transition process and the dynamic logic gate transfer in the dynamic fault tree. When the complexity or scale of system is increasing, the Markov model encountered a problem of state space explosion leading to increase troubles. To solve the above problems, a modular approach is needed. Based on the modular approach, a hybrid fault module was researched in this paper. Firstly, the stackable fault subtree containing complex static/dynamic logic gate is transformed into four common combinational logic gates through preprocessing of the dynamic gate in the module. Then, the complexity of the model was reduced by incorporating four common combinational logic gates and using the binary decision graph to solve variable ordering in the calculation of failure probability of static subtree. Moreover, the calculating process of complex mixed logic gate fault tree can be simplified. An example of the ammonium nitrate/fuel explosive production system for BCZH-15 explosive vehicle was used to verify the feasibility of the presented method.
Luteolin (Lut) is a natural flavonoid mainly extracted from vegetables and fruits. Lut shows great anti-tumor potential in many malignant cancers, which are hindered by poor water solubility and low bioavailability. Peritoneal metastasis is a challenge for colorectal cancer treatment,
usually indicating unfavorable prognosis of patients. Methoxy poly(ethylene glycol)-poly(ε-caprolactone) micelles containing Luteolin (Lut-M) and thermosensitive Pluronic®F127 coated Lut-M (Lut-M-F127) were synthesized and applied in the local therapy of colorectal
cancer. Drug release study of Lut-M-F127 and Lut-M suggested extended drug release, and the release of Lut from Lut-M-F127 was slower than Lut-M. It was also proved that Lut-M-F127 could transit from solution to gel at body temperature. Moreover, both Lut-Free and Lut-M micelles were capable
of inducing tumor cell apoptosis and reducing cell viability in vitro. Our results further demonstrated the therapeutic effect of Lut-M-F127 treatment was much better than that of Lut-M treatment in vivo. Lut-M-F127 has shown strong ability to promote tumor apoptosis, suppress
tumor proliferation and block tumor angiogenesis. In summary, Lut-M-F127 formulation may be a very promising treatment option for peritoneal metastasis in colorectal cancer in the future.
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