The purpose of the present study was to investigate the diagnostic value of T cells spot test (T-Spot TB) combined with interferon-γ (INF-γ) and interleukin-27 (IL-27) in tuberculous pleurisy. Sixty patients with tuberculous pleurisy (observation group) and 60 patients with non-tuberculous pleurisy (control group) were enrolled in this study. T-Spot TB was performed to detect the pleural effusion of two groups of patients. Levels of IFN-γ and IL-27 in serum and pleural effusion were detected by enzyme-linked immunosorbent assay (ELISA). Relative expression of IFN-γ mRNA and IL-27 mRNA in peripheral blood mononuclear cells were detected by RT-PCR. Positive rate of T-Spot TB in observation group was 96.7% (58 cases), which was significantly higher than that in control group (p<0.05). Concentration of INF-γ in pleural effusion of observation group was 468.6±24.8 ng/l, which was significantly higher than that in control group (131.3±18.7 ng/l, p<0.05). Concentration of IL-27 in pleural effusion of observation group was 423.4±37.2 ng/l, which was significantly higher than that in control group (116.2±15.5 ng/l, p<0.05). Concentrations of INF-γ and IL-27 in serum of observation group were 48.2±13.4 and 41.7±10.6 ng/l, respectively, which were significantly higher than those in control group (38.6±11.2 and 35.3±8.4 ng/l, p<0.05). Relative expression levels of INF-γ mRNA and IL-27 mRNA in observation group were significantly higher than those in control group (p<0.05). Therefore, combination of T-Spot TB with INF-γ and IL-27 has significant application value in the clinical diagnosis of tuberculous pleurisy, and should be popularized.
As water trading has become increasingly used to optimize the allocation of water resources, it has become necessary to transfer agricultural water allocations for economic development and ecological environmental uses by way of water rights trading. In this paper, we constructed an example water rights trading model in the arid oasis area of Shihezi Irrigation District (located in Northwest China), using the field investigation method and governmental water management decisions based on the systems theory of the agricultural water savings–economy–ecological environment. Furthermore, focusing on the added industrial value produced by trading water, the value of the ecological services provided by fresh water, the negative value of the reduction in greenhouse gas emissions, and the negative ecological value of reduced fertilizer application, a quantitative analysis was carried out using the Shihezi Irrigation District as an example. The results showed that under the most stringent water resource management plan and with the objective of returning land and reducing water, the irrigation area can save 52,504,500 m3 of surplus water from the agricultural sector, of which 49,879,300 m3 can be reallocated to the industrial sector and 2,625,200 m3 can be reallocated to the ecological sector. Using the water rights transaction method, this regional agricultural water saving could generate an industrial benefit equal to 35,024,300 yuan, an ecosystem service equal to 19,482,200 yuan, and an overall benefit equal to 54,420,500 yuan. The water rights trading model proposed in this paper for an arid oasis areas can provide a reference for other arid areas, helping to achieve the sustainable economic development of the economic and ecological environments in arid oasis areas through water rights trading.
To develop an optimal irrigation and fertilization system for Korla fragrant pear in the Xinjiang region, the effects of water and fertilizer coupling on the quality, yield, irrigation water use efficiency (IWUE), fertilizer partial productivity (PFP), and net profits of Korla fragrant pear under the condition of limited water drip irrigation were studied through field experiments by combining multiple regression analysis and spatial analysis. A comprehensive quality evaluation model of fragrant pear was constructed using the principal component analysis, and 12 quality indices were evaluated comprehensively. The experiment adopted a two-factor crossover design with three irrigation levels (W1: 5250 m3 ha−1, W2: 6750 m3 ha−1, W3: 8250 m3 ha−1), accounting for 60%, 80% and 100% of the ETe (where ETe denotes evapotranspiration under sufficient water supply for crops); four fertilizer application levels (F1: 675 kg ha−1, F2: 750 kg ha−1, F3: 825 kg ha−1, F4: 900 kg ha−1), designated F80%, F90%, F100%, and F110%, respectively; and 12 treatments. The results showed that the overall quality of fragrant pear was improved based on the integrated quality of pear. Four principal components were extracted through the fragrant pear comprehensive quality evaluation model, and their cumulative contribution was 89.977%; the best comprehensive quality was obtained in the W3F2 treatment and the worst comprehensive quality in the W1F1 treatment. The spatial analysis showed that when the irrigation range is 7484–8250 m3 ha−1 and the N-P2O5-K2O fertilization range is (181-223-300)–(200-246-332) kg ha−1, the comprehensive quality, yield, IWUE, PFP, and net profits of fragrant pear can reach > 85% of the maximum value. These results provide a scientific basis for water and fertilizer management of fragrant pear orchard with drip irrigation in Korla, Xinjiang.
A field study in an orchard of Korla fragrant pear tested three levels of irrigation (as varying proportions of evapotranspiration; namely, W1: 70% ETC, W2: 85% ETC, and W3: 100% ETC) and four methods of applying such irrigation; namely, F1: surface drip, F2: subsurface, F3: root zone infiltration, and CK: flood irrigation (as the control or check). The effects of the different treatments were evaluated in terms of plant growth (shoot length and leaf area), fruit yield and quality, and the distribution of water and salt in soil. For a given method of irrigation, soil moisture content, wet-front displacement, the length of new shoots, and leaf area under W3 were significantly higher than those under W1 and W2. The salt content under W3 was also significantly lower than that under W1 and W2, whereas the yield was significantly higher—by 5.89–13.85% compared to that under W2 and by 4.08–13.13% compared to that under W1. For a given volume of irrigation, yield, water-use efficiency, and fruit quality were significantly higher under F3 and F2 than those under F1. Soil water was more uniformly distributed and its content was significantly higher under F3 than the corresponding values under F1 or F2. The salt content of the root zone was the lowest under F3, and most of the soil salt was in soil layers deeper than 80 cm, but there was no significant difference between F3 and F2 (p < 0.05). There were also no significant differences in shoot length and leaf area among the three irrigation methods (F1, F2, and F3) (p < 0.05). Compared to that under F1, root zone infiltration under W1 was 3.61% greater, that under W2 was 6.58% greater, and that under W3 was 5.43% greater. The irrigation water-use efficiency and production factor efficiency for nitrogen under F3 and F2 were significantly higher than those under F1 (p < 0.05). Principal component analysis showed that the comprehensive score for fruit quality under different volumes of irrigation was the highest under W3, was intermediate under W2, and was the lowest under W1. The corresponding ranking of different irrigation methods was F2, F3, F1, and CK. Comprehensive analysis showed that yield, quality, and the efficiency of utilization of water and fertilizer were higher under the combination W3F3 than under any other combination—therefore, irrigation at 100% of evapotranspiration applied through root zone infiltration is recommended for Korla fragrant pear. The research results can provide a theoretical basis for the optimal use of water and for salt control in pear in Korla, Xinjiang.
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