The localization of lipids and the activities of nonspecific esterase, aryl sulfatase and acid phosphatase were studied in different stages of spermatogenesis in rats. In addition, the distribution of acid phosphatase activity was demonstrated electron histochemically. The spermatogenetic cycle was divided into two phases—corresponding to the first and the last four stages of Roosen-Runge-Giesel (RG) classification. Spermatids in the first phase contained abundant endoplasmic reticulum with rosette formation and well developed Golgi apparatus with numerous vesicles. They displayed high activity of hydrolytic enzymes but contained no appreciable amount of lipids. The Sertoli cells contained large lipid granules but showed minimal enzyme activity. During the second phase reduction of the cytoplasm of spermatids with fragmentation of the endoplasmic reticulum and Golgi lamellae, accumulation of lipids, aggregation of ribonucleo-protein particles, formation of residual bodies and marked decrease of enzyme activity were seen. The Sertoli cells contained large mitochondria, well developed endoplasmic reticulum and numerous dense bodies and revealed high activities of hydrolytic enzymes and rapid depletion of lipids. These ultrastructural and histochemical findings suggested an interaction between the Sertoli cells and the developing spermatids which probably contributed to the regulation of spermatogenesis.
The alterations of hepatic microsomal fractions were studied in fasting rats given porphyrogenic doses of allylisopropylacetamide. A single dose of allylisopropylacetamide caused significant enlargement of the liver within 14 hr and in 24 hr the liver was about 49% heavier. Microsomal phospholipids of liver more than doubled in 48 hr after the administration of allylisopropylacetamide. This increase was accounted for principally by an increase in phospholipids of the smooth endoplasmic reticulum. Incorporation of 32P indi cated that the increase in microsomal phospholipids was caused mainly by a reduction of the rate of catabolism rather than by an augmentation of the rate of synthesis. Electron microscopic observations in agreement with the fractionation studies revealed a hypertrophy of the smooth membranes of the endoplasmic reticulum.
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