To evaluate the genetic diversity among 48 genotypes of chickpea comprising cultivars, landraces and internationally developed improved lines genetic distances were evaluated using three different molecular marker techniques: Simple Sequence Repeat (SSR); Start Codon Targeted (SCoT) and Conserved DNA-derived Polymorphism (CDDP). Average polymorphism information content (PIC) for SSR, SCoT and CDDP markers was 0.47, 0.45 and 0.45, respectively, and this revealed that three different marker types were equal for the assessment of diversity amongst genotypes. Cluster analysis for SSR and SCoT divided the genotypes in to three distinct clusters and using CDDP markers data, genotypes grouped in to five clusters. There were positive significant correlation (r = 0.43, P < 0.01) between similarity matrix obtained by SCoT and CDDP. Three different marker techniques showed relatively same pattern of diversity across genotypes and using each marker technique it's obvious that diversity pattern and polymorphism for varieties were higher than that of genotypes, and CDDP had superiority over SCoT and SSR markers. These results suggest that efficiency of SSR, SCOT and CDDP markers was relatively the same in fingerprinting of chickpea genotypes. To our knowledge, this is the first detailed report of using targeted DNA region molecular marker (CDDP) for genetic diversity analysis in chickpea in comparison with SCoT and SSR markers. Overall, our results are able to prove the suitability of SCoT and CDDP markers for genetic diversity analysis in chickpea for their high rates of polymorphism and their potential for genome diversity and germplasm conservation.
Phytohormones play a key role in plant growth and development. The process of plant’s perception and response to abiotic and biotic stresses is controlled mainly by the phytohormones which act as an endogenous messenger in the regulation of the plant’s status. They can be activated by different signaling pathways in response to environmental stresses. Plants respond to environmental stress through interaction of transcription factors with a handful of cis-regulatory elements (CREs). Some examples of cis elements include abscisic acid-responsive element (ABRE), G-box (CACGTG) element, and W-box. In order to investigate the effects of different hormonal stresses which have a key role in response to biotic and abiotic stresses in rice, microarray data was used. Of the available data, 931 genes revealed significant differences in response to different hormonal stresses such as auxin, cytokinin, abcisic acid, ethylene, salicylic acid, and jasmonic acid. The present results showed that 388 genes were up-regulated, and 543 genes were down-regulated. Most of the genes were up-regulated in response to Indole-3-acetic acid (IAA) hormone. Genes Ontology analysis revealed that they respond to various hormones involved in auxin- responsive genes, auxin-activated signaling pathway and cellular responses to environmental stimuli. G-box had the highest number of cis elements involved in hormonal stress and was regulated by auxin signaling and various stresses. Dehydrin was the only gene up-regulated in response to the six hormones. This gene can be activated in response to abiotic and biotic stresses. As such, dehydrin gene can be used in crop breeding programs to increase tolerance to different environmental stresses in various plant species.
Background Plants use escape strategies including premature senescence and leaf reduction to cope in response to drought stress, which in turn reduces plant leaves and photosynthesis. This strategy allows the new generation (seeds) to survive under drought but, plants experience more yield loss during stress condition. The amount of damage caused by drought stress is compensated by the expression of genes involved in regulating leaf aging. Leaf senescence alters the expression of thousands of genes and ultimately affecting grain protein content, grain yield, and nitrogen utilization efficiency. Also, under drought stress, nitrogen in the soil will not become as much available and causes the beginning and acceleration of the senescence process of leaves. The main body of the abstract This review identified proteins signaling and functional proteins involved in senescence. Further, transcription factors and cell wall degradation enzymes (proteases) related to senescence during drought stress were surveyed. We discuss the regulatory pathways of genes as a result of the degradation of proteins during senescence process. Senescence is strongly influenced by plant hormones and environmental factors including the availability of nitrogen. During maturity or drought stress, reduced nitrogen uptake can cause nitrogen to be remobilized from leaves and stems to seeds, eventually leading to leaf senescence. Under these conditions, genes involved in chloroplast degradation and proteases show increased expression. The functional (proteases) and regulatory proteins such as protein kinases and phosphatases as well as transcription factors (AP2/ERF, NAC, WRKY, MYB, and bZIP) are involved in leaf senescence and drought stress. Short conclusion In this review, senescence-associated proteins involved in leaf senescence and regulatory and functional proteins in response to drought stress during grain filling were surveyed. The present study predicts on the role of nitrogen transporters, transcription factors and regulatory genes involved in the late stages of plant growth with the aim of understanding their mechanisms of action during grain filling stage. For a better understanding, the relevant evidence for the balance between grain filling and protein breakdown during grain filling in cereals is presented.
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