Optical microscopy, owing to its noninvasiveness and subcellular resolution, enables in vivo visualization of neuronal structure and function in the physiological context. Optical-sectioning structured illumination microscopy (OS-SIM) is a widefield fluorescence imaging technique that uses structured illumination patterns to encode in-focus structures and optically sections 3D samples. However, its application to in vivo imaging has been limited. In this study, we optimized OS-SIM for in vivo neural imaging. We modified OS-SIM reconstruction algorithms to improve signal-to-noise ratio and correct motion-induced artifacts in live samples. Incorporating an adaptive optics (AO) module to OS-SIM, we found that correcting sample-induced optical aberrations was essential for achieving accurate structural and functional characterizations in vivo. With AO OS-SIM, we demonstrated fast, high-resolution in vivo imaging with optical sectioning for structural imaging of mouse cortical neurons and zebrafish larval motor neurons, and functional imaging of quantal synaptic transmission at Drosophila larval neuromuscular junctions.
Visible light communication (VLC) has emerged as a promising communication method in 6G. However, the development of receiving devices is much slower than that of transmitting devices, limited by materials, structures, and fabrication. In this paper, we propose and fabricate an InGaN/GaN multiple-quantum-well-based vertical-structure micro-LED-based photodetector (μPD) on a Si substrate. A comprehensive comparison of the photoelectrical performance and communication performance of three sizes of μPDs, 10, 50, and 100 μm, is presented. The peak responsivity of all three μPDs is achieved at 400 nm, while the passband full-widths at half maxima are 87, 72, and 78 nm for 10, 50, and 100 μm μPDs, respectively. The − 20 dB cutoff bandwidth is up to 822 MHz for 50 μm μPD. A data rate of 10.14 Gbps is experimentally demonstrated by bit and power loading discrete multitone modulation and the proposed digital pre-equalizer algorithm over 1 m free space utilizing the self-designed 4 × 4 50 μm μPD array as a receiver and a 450 nm laser diode as a transmitter. This is the first time a more than 10 Gbps VLC system has been achieved utilizing a GaN-based micro-PD, to the best of our knowledge. The investigation fully demonstrates the superiority of Si substrates and vertical structures in InGaN/GaN μPDs and shows its great potential for high-speed VLC links beyond 10 Gbps.
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