Background: Primary dysmenorrhea(PD)is the most common gynecologic disorder.Despite the prevalence is high, it is often underdiagnosed,undertreated and normalized even by patients themselves. Guizhi Fuling Formula (GFF) is experientially used for the treatment of PD in a long time. Therefore, the efficiency and potential mechanism are waiting to identify.Methods: We adopted network pharmacology integrated molecular docking strategy in this study.Based on published literatures, the relative compounds of GFF were selected preliminarily. Secondly, the putative targets of PD were obtained by wide-searching DisGeNET, OMIM, Drugbank and GeneCards databases.With protein-protein interaction(PPI) analysis, GO and KEGG pathway enrichment analysis and molecular docking ,we systematically evaluated the relationship of herb ingredients and disease targets.Results: The results showed that 30 ingredients of GFF and 43 hub targets made a difference.Under the further analysis,8 targets(EGFR,AKT1,PTGS2,TNF,ESR1,AHR,CTNNB1,CXCL8) were recognized as key therapeutic targets with excellent binding. The enrichment analyses indicated that the GFF had the potential to influence varieties of biological pathways, especially the pathways in cancer and steroid hormone biosynthesis, which play an important part in the pathogenesis of primary dysmenorrhea.Conclusion: GFF influenced primary dysmenorrhea through the synergistic effect of multiple components, multiple targets, and multiple pathways.This study predictedthe potential mechanism, hope that could made contribution for clinical application and scientific research.
In order to study the quasi black prickly ant extracts antioxidant mechanism, test the completely random method is adopted to 160 rats, and randomly divided into 8 groups, divided into control group, cadmium poisoning group, cadmium and extracts from petroleum ether to black prickly ant group (divided into high, medium and low dose group), cadmium to higher doses of anhydrous ethanol extracts from black prickly ant group, higher dose of petroleum ether to black prickly ant extract group, only higher doses of anhydrous ethanol extracts from black prickly ant group. The mice were fed for 21 days, and the clinical symptoms of the mice were observed every day. On the 22nd day, the liver, spleen, kidney and sexual organs of the mice were dissected and separated, and the organ coefficients of the mice were calculated. After grinding treatment, the contents of MDA, SOD, CAT and T-AOC in kidney and spleen of mice were determined according to the instructions of the kit. Results showed that the mice of the sex organs of liver, spleen, kidney, organ coefficient were elevated compared with control group (P<0.05), cadmium poisoning group mice kidney, spleen MDA levels were elevated compared with control group (P<0.05), cadmium poisoning group mice kidney, spleen SOD, T–AOC,CAT content decreased (P<0.05), The results indicated that the lipid soluble extract of C. nigra had antioxidant ability, and the low and medium dose of lipid soluble extract of C. nigra had less cadmium toxicity than the high doseThe curative effect in mice was remarkable and the anti-inflammatory function was achieved.
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